Identification of a novel myosin heavy chain gene expressed in the rat larynx

Albert L. Merati, Sue C. Bodine, Thecla Bennett, Hak Hyun Jung, Hiroshi Furuta, Allen F. Ryan

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Based on reactivity to antibodies against known myosin heavy chains, expression of a novel fast myosin heavy chain (MHC) gene was suspected in the thyroarytenoid (TA) muscle of the rat larynx. The 3' ends of MHC transcripts in the TA were amplified by RT-PCR using a primer to a highly conserved MHC sequence and to the poly(A) tail. The resultant products were cloned and fourteen PCR products were screened by dot-blotting with oligonucleotides specific for known skeletal muscle MHC genes. A clone that reacted weakly to the 2B oligo was sequenced and found to encode a novel fast MHC transcript, termed 2L, that appears to represent an eighth vertebrate skeletal muscle MHC gene. By homology analysis, the 2L sequence is most similar to the extraocular MHC, suggesting a possible evolutionary relationship between MHCs associated with the branchial arches.

Original languageEnglish
Pages (from-to)153-159
Number of pages7
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1306
Issue number2-3
DOIs
Publication statusPublished - 1996 May 2
Externally publishedYes

Fingerprint

Myosin Heavy Chains
Larynx
Rats
Genes
Skeletal Muscle Myosins
Laryngeal Muscles
Branchial Region
Polymerase Chain Reaction
Arches
Oligonucleotides
Muscle
Vertebrates
Clone Cells
Messenger RNA
Antibodies

Keywords

  • larynx
  • muscle protein
  • myosin evolution
  • myosin heavy chain gene
  • rat

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Cite this

Identification of a novel myosin heavy chain gene expressed in the rat larynx. / Merati, Albert L.; Bodine, Sue C.; Bennett, Thecla; Jung, Hak Hyun; Furuta, Hiroshi; Ryan, Allen F.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1306, No. 2-3, 02.05.1996, p. 153-159.

Research output: Contribution to journalArticle

Merati, Albert L. ; Bodine, Sue C. ; Bennett, Thecla ; Jung, Hak Hyun ; Furuta, Hiroshi ; Ryan, Allen F. / Identification of a novel myosin heavy chain gene expressed in the rat larynx. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 1996 ; Vol. 1306, No. 2-3. pp. 153-159.
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