Identification of a novel myosin heavy chain gene expressed in the rat larynx

Albert L. Merati, Sue C. Bodine, Thecla Bennett, Hak Hyun Jung, Hiroshi Furuta, Allen F. Ryan

    Research output: Contribution to journalArticlepeer-review

    32 Citations (Scopus)


    Based on reactivity to antibodies against known myosin heavy chains, expression of a novel fast myosin heavy chain (MHC) gene was suspected in the thyroarytenoid (TA) muscle of the rat larynx. The 3' ends of MHC transcripts in the TA were amplified by RT-PCR using a primer to a highly conserved MHC sequence and to the poly(A) tail. The resultant products were cloned and fourteen PCR products were screened by dot-blotting with oligonucleotides specific for known skeletal muscle MHC genes. A clone that reacted weakly to the 2B oligo was sequenced and found to encode a novel fast MHC transcript, termed 2L, that appears to represent an eighth vertebrate skeletal muscle MHC gene. By homology analysis, the 2L sequence is most similar to the extraocular MHC, suggesting a possible evolutionary relationship between MHCs associated with the branchial arches.

    Original languageEnglish
    Pages (from-to)153-159
    Number of pages7
    JournalBiochimica et Biophysica Acta - Gene Structure and Expression
    Issue number2-3
    Publication statusPublished - 1996 May 2


    • larynx
    • muscle protein
    • myosin evolution
    • myosin heavy chain gene
    • rat

    ASJC Scopus subject areas

    • Structural Biology
    • Biophysics
    • Biochemistry
    • Genetics


    Dive into the research topics of 'Identification of a novel myosin heavy chain gene expressed in the rat larynx'. Together they form a unique fingerprint.

    Cite this