Identification of a novel protein D3UPCA from Halobacterium salinarum and prediction of its function

Moon Sup Lee, Won A. Joo, Chan Wha Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Halobacterium salinarum is an extremely halophilic archaea, which is able to live in highly saline environments. In a recent study, several halophilic archaea were found to have the ability to biodegrade organic hydrocarbon pollutants, but protein information regarding hydrocarbon degradation and tolerance in halophilic archaea has been relatively rare. In this study, the protein expression profile of H. salinarum cultured under different diesel concentrations (0, 2 and 4%) was investigated by two-dimensional gel electrophoresis. Proteins which increased their expression levels in diesel media were identified using matrix-assisted laser desorption/ ionization-time of flight and electrospray ionization-tandem mass spectrometry analysis. Among these, a protein spot (named D3UPCA) which was up-regulated about nine-fold and found to have COG3388, an uncharacterized protein conserved in archaea, was selected in order to further characterize its functions. The D3UPCA coding gene (named d3upca) was cloned and expressed in Escherichia coli, and purified by the glutathione-S-transferase-fusion method. The function of the protein was estimated using various bioinformatics tools and was predicted to be related to the regulation of transcription and/or translation of genes needed to tolerate stresses associated within the presence of diesel oil.

Original languageEnglish
Pages (from-to)3622-3631
Number of pages10
JournalProteomics
Volume4
Issue number11
DOIs
Publication statusPublished - 2004 Nov 1

Fingerprint

Halobacterium salinarum
Archaea
Proteins
Hydrocarbons
Genes
Electrospray ionization
Electrospray Ionization Mass Spectrometry
Electrophoresis, Gel, Two-Dimensional
Transcription
Bioinformatics
Tandem Mass Spectrometry
Glutathione Transferase
Computational Biology
Electrophoresis
Escherichia coli
Ionization
Mass spectrometry
Desorption
Oils
Lasers

Keywords

  • Electrospray ionization-quadrupole-time of flight mass spectrometry
  • Halobacterium salinarum
  • Matrix-assisted laser desorption/ionization-time of flight
  • Prediction of function
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Identification of a novel protein D3UPCA from Halobacterium salinarum and prediction of its function. / Lee, Moon Sup; Joo, Won A.; Kim, Chan Wha.

In: Proteomics, Vol. 4, No. 11, 01.11.2004, p. 3622-3631.

Research output: Contribution to journalArticle

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