Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells

Samuel Beck, Xun Jin, Jinlong Yin, Sung Hak Kim, Nam Kyung Lee, Se Yeong Oh, Xiong Jin, Min Kook Kim, Eun Bae Kim, Jee Soo Son, Sung Chan Kim, Do Hyun Nam, Se Hyuk Kim, Sang Kee Kang, Hyunggee Kim, Yun Jaie Choi

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Glioma stem cells (GSCs) are presumably major culprits for brain tumor initiation, progression, and recurrence after conventional therapies. Thus, selective targeting and eradication of GSCs may provide a promising and effective therapeutic approach. Here, we isolated a GSC-targeting (GSCT) peptide that demonstrated selective binding affinity for many undifferentiated GSCs using in vitro phage display technology. This GSCT peptide binds to isotypes of Nestin proteins specifically expressed in GSCs, enabling it to target Nestin-positive cells in human glioblastoma tissues. In human glioblastoma tissue specimens, the fluorescence-conjugated GSCT peptide could visualize putative GSC populations, showing its possible use as a diagnostic agent. GSCT peptide is also internalized into undifferentiated GSCs specifically in vitro, and moreover, intravenously injected GSCT peptide effectively penetrated into tissues, specifically accumulated in gliomas that arise from subcutaneous and orthotopic implantation, and predominantly targeted Nestin-positive cells in these tumors. Thus, our GSCT peptide may be useful for the development of more promising therapeutic and diagnostic modalities that target GSCs in brain tumors.

Original languageEnglish
Pages (from-to)8518-8528
Number of pages11
JournalBiomaterials
Volume32
Issue number33
DOIs
Publication statusPublished - 2011 Nov 1

Fingerprint

Nestin
Neoplastic Stem Cells
Stem cells
Brain Neoplasms
Glioma
Peptides
Brain Stem
Brain
Stem Cells
Proteins
Tumors
Tissue
Glioblastoma
Cells
Bacteriophages
Fluorescence
Display devices
Therapeutics
Technology
Recurrence

Keywords

  • Adhesion molecule
  • Brain tumors
  • Glioma stem cells
  • Peptide
  • Phage display

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Beck, S., Jin, X., Yin, J., Kim, S. H., Lee, N. K., Oh, S. Y., ... Choi, Y. J. (2011). Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells. Biomaterials, 32(33), 8518-8528. https://doi.org/10.1016/j.biomaterials.2011.07.048

Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells. / Beck, Samuel; Jin, Xun; Yin, Jinlong; Kim, Sung Hak; Lee, Nam Kyung; Oh, Se Yeong; Jin, Xiong; Kim, Min Kook; Kim, Eun Bae; Son, Jee Soo; Kim, Sung Chan; Nam, Do Hyun; Kim, Se Hyuk; Kang, Sang Kee; Kim, Hyunggee; Choi, Yun Jaie.

In: Biomaterials, Vol. 32, No. 33, 01.11.2011, p. 8518-8528.

Research output: Contribution to journalArticle

Beck, S, Jin, X, Yin, J, Kim, SH, Lee, NK, Oh, SY, Jin, X, Kim, MK, Kim, EB, Son, JS, Kim, SC, Nam, DH, Kim, SH, Kang, SK, Kim, H & Choi, YJ 2011, 'Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells', Biomaterials, vol. 32, no. 33, pp. 8518-8528. https://doi.org/10.1016/j.biomaterials.2011.07.048
Beck, Samuel ; Jin, Xun ; Yin, Jinlong ; Kim, Sung Hak ; Lee, Nam Kyung ; Oh, Se Yeong ; Jin, Xiong ; Kim, Min Kook ; Kim, Eun Bae ; Son, Jee Soo ; Kim, Sung Chan ; Nam, Do Hyun ; Kim, Se Hyuk ; Kang, Sang Kee ; Kim, Hyunggee ; Choi, Yun Jaie. / Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells. In: Biomaterials. 2011 ; Vol. 32, No. 33. pp. 8518-8528.
@article{5d03e560b39a4ebfa77bb444482e2f41,
title = "Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells",
abstract = "Glioma stem cells (GSCs) are presumably major culprits for brain tumor initiation, progression, and recurrence after conventional therapies. Thus, selective targeting and eradication of GSCs may provide a promising and effective therapeutic approach. Here, we isolated a GSC-targeting (GSCT) peptide that demonstrated selective binding affinity for many undifferentiated GSCs using in vitro phage display technology. This GSCT peptide binds to isotypes of Nestin proteins specifically expressed in GSCs, enabling it to target Nestin-positive cells in human glioblastoma tissues. In human glioblastoma tissue specimens, the fluorescence-conjugated GSCT peptide could visualize putative GSC populations, showing its possible use as a diagnostic agent. GSCT peptide is also internalized into undifferentiated GSCs specifically in vitro, and moreover, intravenously injected GSCT peptide effectively penetrated into tissues, specifically accumulated in gliomas that arise from subcutaneous and orthotopic implantation, and predominantly targeted Nestin-positive cells in these tumors. Thus, our GSCT peptide may be useful for the development of more promising therapeutic and diagnostic modalities that target GSCs in brain tumors.",
keywords = "Adhesion molecule, Brain tumors, Glioma stem cells, Peptide, Phage display",
author = "Samuel Beck and Xun Jin and Jinlong Yin and Kim, {Sung Hak} and Lee, {Nam Kyung} and Oh, {Se Yeong} and Xiong Jin and Kim, {Min Kook} and Kim, {Eun Bae} and Son, {Jee Soo} and Kim, {Sung Chan} and Nam, {Do Hyun} and Kim, {Se Hyuk} and Kang, {Sang Kee} and Hyunggee Kim and Choi, {Yun Jaie}",
year = "2011",
month = "11",
day = "1",
doi = "10.1016/j.biomaterials.2011.07.048",
language = "English",
volume = "32",
pages = "8518--8528",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier BV",
number = "33",

}

TY - JOUR

T1 - Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells

AU - Beck, Samuel

AU - Jin, Xun

AU - Yin, Jinlong

AU - Kim, Sung Hak

AU - Lee, Nam Kyung

AU - Oh, Se Yeong

AU - Jin, Xiong

AU - Kim, Min Kook

AU - Kim, Eun Bae

AU - Son, Jee Soo

AU - Kim, Sung Chan

AU - Nam, Do Hyun

AU - Kim, Se Hyuk

AU - Kang, Sang Kee

AU - Kim, Hyunggee

AU - Choi, Yun Jaie

PY - 2011/11/1

Y1 - 2011/11/1

N2 - Glioma stem cells (GSCs) are presumably major culprits for brain tumor initiation, progression, and recurrence after conventional therapies. Thus, selective targeting and eradication of GSCs may provide a promising and effective therapeutic approach. Here, we isolated a GSC-targeting (GSCT) peptide that demonstrated selective binding affinity for many undifferentiated GSCs using in vitro phage display technology. This GSCT peptide binds to isotypes of Nestin proteins specifically expressed in GSCs, enabling it to target Nestin-positive cells in human glioblastoma tissues. In human glioblastoma tissue specimens, the fluorescence-conjugated GSCT peptide could visualize putative GSC populations, showing its possible use as a diagnostic agent. GSCT peptide is also internalized into undifferentiated GSCs specifically in vitro, and moreover, intravenously injected GSCT peptide effectively penetrated into tissues, specifically accumulated in gliomas that arise from subcutaneous and orthotopic implantation, and predominantly targeted Nestin-positive cells in these tumors. Thus, our GSCT peptide may be useful for the development of more promising therapeutic and diagnostic modalities that target GSCs in brain tumors.

AB - Glioma stem cells (GSCs) are presumably major culprits for brain tumor initiation, progression, and recurrence after conventional therapies. Thus, selective targeting and eradication of GSCs may provide a promising and effective therapeutic approach. Here, we isolated a GSC-targeting (GSCT) peptide that demonstrated selective binding affinity for many undifferentiated GSCs using in vitro phage display technology. This GSCT peptide binds to isotypes of Nestin proteins specifically expressed in GSCs, enabling it to target Nestin-positive cells in human glioblastoma tissues. In human glioblastoma tissue specimens, the fluorescence-conjugated GSCT peptide could visualize putative GSC populations, showing its possible use as a diagnostic agent. GSCT peptide is also internalized into undifferentiated GSCs specifically in vitro, and moreover, intravenously injected GSCT peptide effectively penetrated into tissues, specifically accumulated in gliomas that arise from subcutaneous and orthotopic implantation, and predominantly targeted Nestin-positive cells in these tumors. Thus, our GSCT peptide may be useful for the development of more promising therapeutic and diagnostic modalities that target GSCs in brain tumors.

KW - Adhesion molecule

KW - Brain tumors

KW - Glioma stem cells

KW - Peptide

KW - Phage display

UR - http://www.scopus.com/inward/record.url?scp=80052964101&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052964101&partnerID=8YFLogxK

U2 - 10.1016/j.biomaterials.2011.07.048

DO - 10.1016/j.biomaterials.2011.07.048

M3 - Article

VL - 32

SP - 8518

EP - 8528

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 33

ER -