Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells

Samuel Beck, Xun Jin, Jinlong Yin, Sung Hak Kim, Nam Kyung Lee, Se Yeong Oh, Xiong Jin, Min Kook Kim, Eun Bae Kim, Jee Soo Son, Sung Chan Kim, Do Hyun Nam, Se Hyuk Kim, Sang Kee Kang, Hyunggee Kim, Yun Jaie Choi

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30 Citations (Scopus)

Abstract

Glioma stem cells (GSCs) are presumably major culprits for brain tumor initiation, progression, and recurrence after conventional therapies. Thus, selective targeting and eradication of GSCs may provide a promising and effective therapeutic approach. Here, we isolated a GSC-targeting (GSCT) peptide that demonstrated selective binding affinity for many undifferentiated GSCs using in vitro phage display technology. This GSCT peptide binds to isotypes of Nestin proteins specifically expressed in GSCs, enabling it to target Nestin-positive cells in human glioblastoma tissues. In human glioblastoma tissue specimens, the fluorescence-conjugated GSCT peptide could visualize putative GSC populations, showing its possible use as a diagnostic agent. GSCT peptide is also internalized into undifferentiated GSCs specifically in vitro, and moreover, intravenously injected GSCT peptide effectively penetrated into tissues, specifically accumulated in gliomas that arise from subcutaneous and orthotopic implantation, and predominantly targeted Nestin-positive cells in these tumors. Thus, our GSCT peptide may be useful for the development of more promising therapeutic and diagnostic modalities that target GSCs in brain tumors.

Original languageEnglish
Pages (from-to)8518-8528
Number of pages11
JournalBiomaterials
Volume32
Issue number33
DOIs
Publication statusPublished - 2011 Nov 1

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Keywords

  • Adhesion molecule
  • Brain tumors
  • Glioma stem cells
  • Peptide
  • Phage display

ASJC Scopus subject areas

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics

Cite this

Beck, S., Jin, X., Yin, J., Kim, S. H., Lee, N. K., Oh, S. Y., Jin, X., Kim, M. K., Kim, E. B., Son, J. S., Kim, S. C., Nam, D. H., Kim, S. H., Kang, S. K., Kim, H., & Choi, Y. J. (2011). Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells. Biomaterials, 32(33), 8518-8528. https://doi.org/10.1016/j.biomaterials.2011.07.048