Identification of antifungal niphimycin from Streptomyces sp. KP6107 by screening based on adenylate kinase assay

Hye Yoon Kim, Jeong Do Kim, Jin Sung Hong, Jong Hyun Ham, Beom Seok Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Microbial culture extracts are used for natural product screening to find antifungal lead compounds. A microbial culture extract library was constructed using 343 actinomycete isolates to examine the value of the adenylate kinase (AK) assay for screening to identify antifungal metabolites that disrupt cell integrity in plant pathogenic fungi. A culture extract of Streptomyces sp. strain KP6107 lysed cells of Fusarium oxysporum f.sp. lycopersici which resulted in high AK activity. The active ingredient N-1 was purified from the culture extract using various chromatographic procedures and identified to be the guanidyl-polyol macrolide antibiotic, niphimycin, which is a potent fungal cell membrane disruptor. Niphimycin showed broad-spectrum antifungal activity against Alternaria mali, Aspergillus oryzae, Colletotrichum coccodes, Colletotrichum gloeosporioides, Cercospora canescens, Cylindrocarpon destructans, F. oxysporum f.sp. cucumerinum, F. oxysporum f.sp. lycopersici, and Rhizoctonia solani at concentrations of 8-64μgml-1. Anthracnose development in pepper plants was completely inhibited by treatment with 50μgml-1 niphimycin, which was as effective as chlorothalonil. These results show that the AK assay is an efficient and selective tool in screening for cell membrane/wall disruptors of plant pathogenic fungi.

Original languageEnglish
Pages (from-to)581-589
Number of pages9
JournalJournal of Basic Microbiology
Volume53
Issue number7
DOIs
Publication statusPublished - 2013 Jul 1

Fingerprint

Adenylate Kinase
Streptomyces
Colletotrichum
tetrachloroisophthalonitrile
Fungi
Cell Membrane
Rhizoctonia
Mali
Aspergillus oryzae
Alternaria
Actinobacteria
Macrolides
Fusarium
Biological Products
Cell Wall
Libraries
Anti-Bacterial Agents
niphimycin

Keywords

  • Adenylate kinase assay
  • Guanidyl-polyol macrolide
  • Niphimycin
  • Plant disease control
  • Streptomyces

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Identification of antifungal niphimycin from Streptomyces sp. KP6107 by screening based on adenylate kinase assay. / Kim, Hye Yoon; Kim, Jeong Do; Hong, Jin Sung; Ham, Jong Hyun; Kim, Beom Seok.

In: Journal of Basic Microbiology, Vol. 53, No. 7, 01.07.2013, p. 581-589.

Research output: Contribution to journalArticle

Kim, Hye Yoon ; Kim, Jeong Do ; Hong, Jin Sung ; Ham, Jong Hyun ; Kim, Beom Seok. / Identification of antifungal niphimycin from Streptomyces sp. KP6107 by screening based on adenylate kinase assay. In: Journal of Basic Microbiology. 2013 ; Vol. 53, No. 7. pp. 581-589.
@article{7e0b0a4378a3490da139929d04e62d67,
title = "Identification of antifungal niphimycin from Streptomyces sp. KP6107 by screening based on adenylate kinase assay",
abstract = "Microbial culture extracts are used for natural product screening to find antifungal lead compounds. A microbial culture extract library was constructed using 343 actinomycete isolates to examine the value of the adenylate kinase (AK) assay for screening to identify antifungal metabolites that disrupt cell integrity in plant pathogenic fungi. A culture extract of Streptomyces sp. strain KP6107 lysed cells of Fusarium oxysporum f.sp. lycopersici which resulted in high AK activity. The active ingredient N-1 was purified from the culture extract using various chromatographic procedures and identified to be the guanidyl-polyol macrolide antibiotic, niphimycin, which is a potent fungal cell membrane disruptor. Niphimycin showed broad-spectrum antifungal activity against Alternaria mali, Aspergillus oryzae, Colletotrichum coccodes, Colletotrichum gloeosporioides, Cercospora canescens, Cylindrocarpon destructans, F. oxysporum f.sp. cucumerinum, F. oxysporum f.sp. lycopersici, and Rhizoctonia solani at concentrations of 8-64μgml-1. Anthracnose development in pepper plants was completely inhibited by treatment with 50μgml-1 niphimycin, which was as effective as chlorothalonil. These results show that the AK assay is an efficient and selective tool in screening for cell membrane/wall disruptors of plant pathogenic fungi.",
keywords = "Adenylate kinase assay, Guanidyl-polyol macrolide, Niphimycin, Plant disease control, Streptomyces",
author = "Kim, {Hye Yoon} and Kim, {Jeong Do} and Hong, {Jin Sung} and Ham, {Jong Hyun} and Kim, {Beom Seok}",
year = "2013",
month = "7",
day = "1",
doi = "10.1002/jobm.201200045",
language = "English",
volume = "53",
pages = "581--589",
journal = "Journal of Basic Microbiology",
issn = "0233-111X",
publisher = "Wiley-VCH Verlag",
number = "7",

}

TY - JOUR

T1 - Identification of antifungal niphimycin from Streptomyces sp. KP6107 by screening based on adenylate kinase assay

AU - Kim, Hye Yoon

AU - Kim, Jeong Do

AU - Hong, Jin Sung

AU - Ham, Jong Hyun

AU - Kim, Beom Seok

PY - 2013/7/1

Y1 - 2013/7/1

N2 - Microbial culture extracts are used for natural product screening to find antifungal lead compounds. A microbial culture extract library was constructed using 343 actinomycete isolates to examine the value of the adenylate kinase (AK) assay for screening to identify antifungal metabolites that disrupt cell integrity in plant pathogenic fungi. A culture extract of Streptomyces sp. strain KP6107 lysed cells of Fusarium oxysporum f.sp. lycopersici which resulted in high AK activity. The active ingredient N-1 was purified from the culture extract using various chromatographic procedures and identified to be the guanidyl-polyol macrolide antibiotic, niphimycin, which is a potent fungal cell membrane disruptor. Niphimycin showed broad-spectrum antifungal activity against Alternaria mali, Aspergillus oryzae, Colletotrichum coccodes, Colletotrichum gloeosporioides, Cercospora canescens, Cylindrocarpon destructans, F. oxysporum f.sp. cucumerinum, F. oxysporum f.sp. lycopersici, and Rhizoctonia solani at concentrations of 8-64μgml-1. Anthracnose development in pepper plants was completely inhibited by treatment with 50μgml-1 niphimycin, which was as effective as chlorothalonil. These results show that the AK assay is an efficient and selective tool in screening for cell membrane/wall disruptors of plant pathogenic fungi.

AB - Microbial culture extracts are used for natural product screening to find antifungal lead compounds. A microbial culture extract library was constructed using 343 actinomycete isolates to examine the value of the adenylate kinase (AK) assay for screening to identify antifungal metabolites that disrupt cell integrity in plant pathogenic fungi. A culture extract of Streptomyces sp. strain KP6107 lysed cells of Fusarium oxysporum f.sp. lycopersici which resulted in high AK activity. The active ingredient N-1 was purified from the culture extract using various chromatographic procedures and identified to be the guanidyl-polyol macrolide antibiotic, niphimycin, which is a potent fungal cell membrane disruptor. Niphimycin showed broad-spectrum antifungal activity against Alternaria mali, Aspergillus oryzae, Colletotrichum coccodes, Colletotrichum gloeosporioides, Cercospora canescens, Cylindrocarpon destructans, F. oxysporum f.sp. cucumerinum, F. oxysporum f.sp. lycopersici, and Rhizoctonia solani at concentrations of 8-64μgml-1. Anthracnose development in pepper plants was completely inhibited by treatment with 50μgml-1 niphimycin, which was as effective as chlorothalonil. These results show that the AK assay is an efficient and selective tool in screening for cell membrane/wall disruptors of plant pathogenic fungi.

KW - Adenylate kinase assay

KW - Guanidyl-polyol macrolide

KW - Niphimycin

KW - Plant disease control

KW - Streptomyces

UR - http://www.scopus.com/inward/record.url?scp=84880422365&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880422365&partnerID=8YFLogxK

U2 - 10.1002/jobm.201200045

DO - 10.1002/jobm.201200045

M3 - Article

C2 - 22915202

AN - SCOPUS:84880422365

VL - 53

SP - 581

EP - 589

JO - Journal of Basic Microbiology

JF - Journal of Basic Microbiology

SN - 0233-111X

IS - 7

ER -