Identification of Biomarkers Associated with Liver Metastasis Progression from Colorectal Cancer Using Exosomal RNA Profiling

Soohyeon Lee, Young Soo Park, Jwa Hoon Kim, Ah Reum Lim, Myung Han Hyun, Boyeon Kim, Jong Won Lee, Saet Byeol Lee, Yeul Hong Kim

Research output: Contribution to journalArticlepeer-review

Abstract

This study aimed to identify novel biomarkers for metastatic colorectal cancer progression using exosomal RNA expression profiling. The exosomal RNA expression profiles of 54 patients with mCRC were investigated. Exosomal RNA profiling was performed at the time of relapse immediately before metastasectomy and cancer recurrence or progression after metastasectomy. The up- and down-regulated RNA expression profiles were screened and analyzed using H-cluster, principle component analysis and gene ontology. The tissue expression profile of the liver metastases was compared with the GSE 41258 set using GSEA tools. We identified two distinctive biological process gene sets (IFNA and PCDB families) related to metastatic progression. The interferon-α response gene set was enriched, especially when the tumor volume was ≥1 cm3. CXCL10, CXCL11 and SAMD 9 mRNA were highly expressed in the plasma exosome samples of patients with mCRC to the liver. Furthermore, high expression of CXCL10 but not CXCL11 or SAMD9 was associated with a poor prognosis and shorter progression-free survival. Conclusions: Cancer-derived exosomal CXCL10 may be a novel biomarker for liver metastasis of mCRC and a potential target for the prevention and treatment of mCRC with liver metastasis.

Original languageEnglish
Article number4723
JournalCancers
Volume14
Issue number19
DOIs
Publication statusPublished - 2022 Oct

Keywords

  • exosomal RNA
  • liquid biopsy
  • liver metastasis
  • metastatic colorectal cancer
  • therapy resistance

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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