Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins

Jae Wook Yoon, Jae Yeon Jung, Hyun Jung Chung, Mi Ryung Kim, Chan Wha Kim, Seung Taik Lim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Starch granule-associated proteins (SGAPs) were extracted from various starches and analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with silver staining. The SDS-PAGE results showed that granule-bound starch synthase (GBSS) of approximately 60 kDa was found in most starches, except waxy-type starches. Starches exhibited the presence of proteins specific to the botanical origin, including those of 22 kDa for maize; 160 and 98 kDa for potato; 140, 115, 90, and 80 kDa for wheat. These proteins could be detected from noodles prepared with the corresponding starches. The detection of the specific proteins by SDS-PAGE may be used to identify the origin of starch incorporated in various foods and industrial products.

Original languageEnglish
Pages (from-to)321-326
Number of pages6
JournalJournal of Cereal Science
Volume52
Issue number2
DOIs
Publication statusPublished - 2010 Sep 1

Fingerprint

Electrophoresis
starch granules
Sodium Dodecyl Sulfate
Starch
polyacrylamide gel electrophoresis
Polyacrylamide Gel Electrophoresis
starch
Proteins
proteins
Starch Synthase
starch synthase
noodles
Silver Staining
Solanum tuberosum
polyacrylamide gels
granules
Silver
Triticum
Zea mays
potatoes

Keywords

  • Botanical origin
  • SDS-PAGE
  • Starch granule-associated protein (SGAP)
  • Starch noodle

ASJC Scopus subject areas

  • Food Science
  • Biochemistry

Cite this

Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins. / Yoon, Jae Wook; Jung, Jae Yeon; Chung, Hyun Jung; Kim, Mi Ryung; Kim, Chan Wha; Lim, Seung Taik.

In: Journal of Cereal Science, Vol. 52, No. 2, 01.09.2010, p. 321-326.

Research output: Contribution to journalArticle

Yoon, Jae Wook ; Jung, Jae Yeon ; Chung, Hyun Jung ; Kim, Mi Ryung ; Kim, Chan Wha ; Lim, Seung Taik. / Identification of botanical origin of starches by SDS-PAGE analysis of starch granule-associated proteins. In: Journal of Cereal Science. 2010 ; Vol. 52, No. 2. pp. 321-326.
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