Identification of candidate genes for the seed coat colour change in a Brachypodium distachyon mutant induced by gamma radiation using whole-genome re-sequencing

Man Bo Lee, Dae Yeon Kim, Yong Weon Seo, F. Belzile

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Brachypodium distachyon has been proposed as a model plant for agriculturally important cereal crops such as wheat and barley. Seed coat colour change from brown-red to yellow was observed in a mutant line (142-3) of B. distachyon, which was induced by chronic gamma radiation. In addition, dwarf phenotypes were observed in each of the lines 142-3, 421-2, and 1376-1. To identify causal mutations for the seed coat colour change, the three mutant lines and the wild type were subjected to whole-genome re-sequencing. After removing natural variations, 906, 1057, and 978 DNA polymorphisms were detected in 142-3, 421-2, and 1376-1, respectively. A total of 13 high-risk DNA polymorphisms were identified in mutant 142-3. Based on a comparison with DNA polymorphisms in 421-2 and 1376-1, candidate causal mutations for the seed coat colour change in 142-3 were selected. In the two independent Arabidopsis thaliana lines carrying T-DNA insertions in the AtCHI, seed colour change was observed. We propose a frameshift mutation in BdCHI1 as a causal mutation responsible for seed colour change in 142-3. The DNA polymorphism information for these mutant lines can be utilized for functional genomics in B. distachyon and cereal crops.

Original languageEnglish
Pages (from-to)581-587
Number of pages7
JournalGenome
Volume60
Issue number7
DOIs
Publication statusPublished - 2017

Fingerprint

Brachypodium
Gamma Rays
Genetic Association Studies
Seeds
Color
Genome
DNA
Mutation
Frameshift Mutation
Hordeum
Genomics
Arabidopsis
Triticum
Phenotype

Keywords

  • Brachypodium distachyon
  • Gamma radiation
  • InDel
  • Seed coat colour
  • SNP

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

Cite this

Identification of candidate genes for the seed coat colour change in a Brachypodium distachyon mutant induced by gamma radiation using whole-genome re-sequencing. / Lee, Man Bo; Kim, Dae Yeon; Seo, Yong Weon; Belzile, F.

In: Genome, Vol. 60, No. 7, 2017, p. 581-587.

Research output: Contribution to journalArticle

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