Identification of differentially expressed genes by gabapentin in cultured dorsal root ganglion in a rat neuropathic pain model

Ji Hye Heo, Seung Ha Lee, Kyung Ha Chang, Eun Hye Han, Seung Kwan Lee, DalWoong Choi, Suhng Wook Kim

Research output: Contribution to journalArticle

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Abstract

Neuropathic pain is a chronic pain disorder caused by nervous system lesions as a direct consequence of a lesion or by disease of the portions of the nervous system that normally signal pain. The spinal nerve ligation (SNL) model in rats that reflect some components of clinical pain have played a crucial role in the understanding of neuropathic pain. To investigate the direct effects of gabapentin on differential gene expression in cultured dorsal root ganglion (DRG) cells of SNL model rats, we performed a differential display reverse transcription-polymerase chain reaction analysis with random priming approach using annealing control primer. Genes encoding metallothionein 1a, transforming growth factor-β1 and palmitoyl-protein thioesterase-2 were up-regulated in gabapentin-treated DRG cells of SNL model rats. The functional roles of these differentially expressed genes were previously suggested as neuroprotective genes. Further study of these genes is expected to reveal potential targets of gabapentin.

Original languageEnglish
Pages (from-to)126-131
Number of pages6
JournalBiomolecules and Therapeutics
Volume21
Issue number2
DOIs
Publication statusPublished - 2013 Apr 9

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Keywords

  • Dorsal root ganglion
  • Gabapentin
  • Neuropathic pain
  • Spinal nerve ligation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Drug Discovery
  • Pharmacology

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