Abstract
Flower development is a complex process mediated by a cascade of transcriptional regulation and signal transduction. Differentially expressed genes in two different developmental stages of carnation flowers were isolated. For this purpose, the suppression subtractive hybridization (SSH) technique followed by the differential hybridization screening was employed to identify rarely transcribed flower maturation-inducible genes. Using the reverse Northern blot analysis we screened 85 positive clones from a total 274 clones obtained by SSH. Among these 85 cDNAs, 60 genes showed obvious distinction of signal intensity. Thirty-five out of 60 clones were analyzed by Northern blot, and 60% (21 of 35) of the clones revealed the true positives of flower maturation-related genes, but others were not certain. Thirteen clones (CFMI-3, 5, 6, 8, 9, 10, 11, 16, 38, 61, 230, 237, 263) were only expressed in mature flowers. None of 13 clones exhibited any visible expression in the driver populations, but were distinctly induced during flower maturation (tester populations). However, the other eight clones (CFMI-7, 14, 49, 66, 203, 205, 243, 388) showed a basal level of expression in the flower bud and increased expression in the mature flower.
| Original language | English |
|---|---|
| Pages (from-to) | 291-297 |
| Number of pages | 7 |
| Journal | Plant Science |
| Volume | 165 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2003 Aug 1 |
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Keywords
- Differentially expressed genes
- Expressed sequence tags (ESTs)
- Flower maturation-inducible genes
- Suppression subtractive hybridization
ASJC Scopus subject areas
- Plant Science
- Biochemistry
- Biotechnology
Cite this
Identification of differentially expressed genes during flower development in carnation (Dianthus caryophyllus). / Ok, Sung Han; Park, Hyun Mi; Kim, Ji Young; Bahn, Sung Chul; Bae, Jung Myung; Suh, Mi Chung; Jeung, Ji Ung; Kim, Kyung Nam; Shin, Jeong Sheop.
In: Plant Science, Vol. 165, No. 2, 01.08.2003, p. 291-297.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of differentially expressed genes during flower development in carnation (Dianthus caryophyllus)
AU - Ok, Sung Han
AU - Park, Hyun Mi
AU - Kim, Ji Young
AU - Bahn, Sung Chul
AU - Bae, Jung Myung
AU - Suh, Mi Chung
AU - Jeung, Ji Ung
AU - Kim, Kyung Nam
AU - Shin, Jeong Sheop
PY - 2003/8/1
Y1 - 2003/8/1
N2 - Flower development is a complex process mediated by a cascade of transcriptional regulation and signal transduction. Differentially expressed genes in two different developmental stages of carnation flowers were isolated. For this purpose, the suppression subtractive hybridization (SSH) technique followed by the differential hybridization screening was employed to identify rarely transcribed flower maturation-inducible genes. Using the reverse Northern blot analysis we screened 85 positive clones from a total 274 clones obtained by SSH. Among these 85 cDNAs, 60 genes showed obvious distinction of signal intensity. Thirty-five out of 60 clones were analyzed by Northern blot, and 60% (21 of 35) of the clones revealed the true positives of flower maturation-related genes, but others were not certain. Thirteen clones (CFMI-3, 5, 6, 8, 9, 10, 11, 16, 38, 61, 230, 237, 263) were only expressed in mature flowers. None of 13 clones exhibited any visible expression in the driver populations, but were distinctly induced during flower maturation (tester populations). However, the other eight clones (CFMI-7, 14, 49, 66, 203, 205, 243, 388) showed a basal level of expression in the flower bud and increased expression in the mature flower.
AB - Flower development is a complex process mediated by a cascade of transcriptional regulation and signal transduction. Differentially expressed genes in two different developmental stages of carnation flowers were isolated. For this purpose, the suppression subtractive hybridization (SSH) technique followed by the differential hybridization screening was employed to identify rarely transcribed flower maturation-inducible genes. Using the reverse Northern blot analysis we screened 85 positive clones from a total 274 clones obtained by SSH. Among these 85 cDNAs, 60 genes showed obvious distinction of signal intensity. Thirty-five out of 60 clones were analyzed by Northern blot, and 60% (21 of 35) of the clones revealed the true positives of flower maturation-related genes, but others were not certain. Thirteen clones (CFMI-3, 5, 6, 8, 9, 10, 11, 16, 38, 61, 230, 237, 263) were only expressed in mature flowers. None of 13 clones exhibited any visible expression in the driver populations, but were distinctly induced during flower maturation (tester populations). However, the other eight clones (CFMI-7, 14, 49, 66, 203, 205, 243, 388) showed a basal level of expression in the flower bud and increased expression in the mature flower.
KW - Differentially expressed genes
KW - Expressed sequence tags (ESTs)
KW - Flower maturation-inducible genes
KW - Suppression subtractive hybridization
UR - http://www.scopus.com/inward/record.url?scp=0038794821&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0038794821&partnerID=8YFLogxK
U2 - 10.1016/S0168-9452(03)00015-3
DO - 10.1016/S0168-9452(03)00015-3
M3 - Article
VL - 165
SP - 291
EP - 297
JO - Plant Science
JF - Plant Science
SN - 0168-9452
IS - 2
ER -