Abstract
Mesenchymal stem cells (MSCs) have greater potential for immediate clinical and toxicological applications, due to their ability to self-renew, proliferate, and differentiate into a variety of cell types. To identify novel candidate genes that were specifically expressed during transdifferentiation of human MSCs to neuronal cells, we performed a differential expression analysis with random priming approach using annealing control primer-based differential display reverse transcription-polymerase chain reaction approach. We identified genes for acyl-CoA thioesterase, tissue inhibitor of metalloproteinases-1, brain glycogen phosphorylase, ubiquitin C-terminal hydrolase and aldehyde reductase were up-regualted, whereas genes for transgelin and heparan sulfate proteoglycan were down-regulated in MSC-derived neurons. These differentially expressed genes may have potential role in regulation of neurogenesis. This study could be applied to environmental toxicology in the field of testing the toxicity of a chemical or a physical agent.
| Original language | English |
|---|---|
| Pages (from-to) | 15-19 |
| Number of pages | 5 |
| Journal | Toxicological Research |
| Volume | 26 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2010 Mar 1 |
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Keywords
- Differentially expressed genes
- Mesenchymal stem cells
- Neuron
ASJC Scopus subject areas
- Toxicology
- Health, Toxicology and Mutagenesis
Cite this
Identification of differentially expressed genes in human mesenchymal stem cell-derived neurons. / Heo, Ji Hye; Cho, Kyung Jin; Choi, DalWoong; Kim, Suhng Wook.
In: Toxicological Research, Vol. 26, No. 1, 01.03.2010, p. 15-19.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of differentially expressed genes in human mesenchymal stem cell-derived neurons
AU - Heo, Ji Hye
AU - Cho, Kyung Jin
AU - Choi, DalWoong
AU - Kim, Suhng Wook
PY - 2010/3/1
Y1 - 2010/3/1
N2 - Mesenchymal stem cells (MSCs) have greater potential for immediate clinical and toxicological applications, due to their ability to self-renew, proliferate, and differentiate into a variety of cell types. To identify novel candidate genes that were specifically expressed during transdifferentiation of human MSCs to neuronal cells, we performed a differential expression analysis with random priming approach using annealing control primer-based differential display reverse transcription-polymerase chain reaction approach. We identified genes for acyl-CoA thioesterase, tissue inhibitor of metalloproteinases-1, brain glycogen phosphorylase, ubiquitin C-terminal hydrolase and aldehyde reductase were up-regualted, whereas genes for transgelin and heparan sulfate proteoglycan were down-regulated in MSC-derived neurons. These differentially expressed genes may have potential role in regulation of neurogenesis. This study could be applied to environmental toxicology in the field of testing the toxicity of a chemical or a physical agent.
AB - Mesenchymal stem cells (MSCs) have greater potential for immediate clinical and toxicological applications, due to their ability to self-renew, proliferate, and differentiate into a variety of cell types. To identify novel candidate genes that were specifically expressed during transdifferentiation of human MSCs to neuronal cells, we performed a differential expression analysis with random priming approach using annealing control primer-based differential display reverse transcription-polymerase chain reaction approach. We identified genes for acyl-CoA thioesterase, tissue inhibitor of metalloproteinases-1, brain glycogen phosphorylase, ubiquitin C-terminal hydrolase and aldehyde reductase were up-regualted, whereas genes for transgelin and heparan sulfate proteoglycan were down-regulated in MSC-derived neurons. These differentially expressed genes may have potential role in regulation of neurogenesis. This study could be applied to environmental toxicology in the field of testing the toxicity of a chemical or a physical agent.
KW - Differentially expressed genes
KW - Mesenchymal stem cells
KW - Neuron
UR - http://www.scopus.com/inward/record.url?scp=84863134805&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863134805&partnerID=8YFLogxK
U2 - 10.5487/TR.2010.26.1.015
DO - 10.5487/TR.2010.26.1.015
M3 - Article
AN - SCOPUS:84863134805
VL - 26
SP - 15
EP - 19
JO - Toxicological Research
JF - Toxicological Research
SN - 1976-8257
IS - 1
ER -