Identification of differentially expressed genes in human mesenchymal stem cell-derived neurons

Ji Hye Heo, Kyung Jin Cho, DalWoong Choi, Suhng Wook Kim

Research output: Contribution to journalArticle

Abstract

Mesenchymal stem cells (MSCs) have greater potential for immediate clinical and toxicological applications, due to their ability to self-renew, proliferate, and differentiate into a variety of cell types. To identify novel candidate genes that were specifically expressed during transdifferentiation of human MSCs to neuronal cells, we performed a differential expression analysis with random priming approach using annealing control primer-based differential display reverse transcription-polymerase chain reaction approach. We identified genes for acyl-CoA thioesterase, tissue inhibitor of metalloproteinases-1, brain glycogen phosphorylase, ubiquitin C-terminal hydrolase and aldehyde reductase were up-regualted, whereas genes for transgelin and heparan sulfate proteoglycan were down-regulated in MSC-derived neurons. These differentially expressed genes may have potential role in regulation of neurogenesis. This study could be applied to environmental toxicology in the field of testing the toxicity of a chemical or a physical agent.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalToxicological Research
Volume26
Issue number1
DOIs
Publication statusPublished - 2010 Mar 1

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Keywords

  • Differentially expressed genes
  • Mesenchymal stem cells
  • Neuron

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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