Identification of EDIL3 on extracellular vesicles involved in breast cancer cell invasion

Jeong Eun Lee, Pyong Gon Moon, Young Eun Cho, Young Bum Kim, In-San Kim, Hoyong Park, Moon Chang Baek

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Cancer cell-derived extracellular vesicles have been linked to the pathogenesis of various cancers; however, the role of extracellular vesicles in tumorigenesis remains unclear. To identify extracellular vesicle proteins involved in cancer metastasis, quantitative proteomic analyses were performed on extracellular vesicles derived from two representative breast cancer cell lines: the less invasive MCF-7 and the invasive MDA-MB-231. Proteomic analysis allowed for the identification of 270 proteins in the extracellular vesicles. Here we report a new function of EDIL3 on extracellular vesicles, which are sufficient for enhancement of cell invasion and for acceleration of lung metastasis in vivo. This invasion is most likely mediated via the integrin-FAK signaling cascade in breast cancer cells. However, these effects are suppressed when EDIL3 is inactivated, providing evidence for a critical role of EDIL3 in development of cancer. Consistently, in human patients with metastatic breast cancer, the levels of EDIL3 on circulating extracellular vesicles are significantly elevated. This information is a remarkable breakthrough in understanding of the molecular mechanism underlying metastasis of breast cancer as well as in the research for cancer biomarkers using circulating extracellular vesicles. Furthermore, targeting EDIL3 on extracellular vesicles may lead to a new therapeutic option for treatment of breast cancer.

Original languageEnglish
Pages (from-to)17-28
Number of pages12
JournalJournal of Proteomics
Volume131
DOIs
Publication statusPublished - 2016 Jan 10
Externally publishedYes

Keywords

  • Breast cancer
  • Cancer biomarker
  • Cancer invasion
  • Extracellular vesicles
  • Integrin-FAK signaling

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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