Identification of essential amino acid residues for catalytic activity and thermostability of novel chitosanase by site-directed mutagenesis

H. G. Yoon, H. Y. Kim, Young Hee Lim, H. K. Kim, D. H. Shin, B. S. Hong, H. Y. Cho

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The functional importance of a conserved region in a novel chitosanase from Bacillus sp. CK4 was investigated. Each of the three carboxylic amino acid residues (Glu-50, Glu-62, and Asp-66) was changed to Asp and Gln or Asn and Glu by site-directed mutagenesis, respectively. The Asp-66→Asn and Asp-66→Glu mutation remarkably decreased kinetic parameters such as Vmax and kcat to approximately 1/1,000 those of the wild-type enzyme, indicating that the Asp-66 residue was essential for catalysis. The thermostable chitosanase contains three Cys residues at positions 49, 72, and 211. The Cys-49→Ser/Tyr and Cys-72→Ser/Tyr mutant enzymes were as stable to thermal inactivation and denaturating agents as the wild-type enzyme. However, the half-life of the Cys-211→Ser/Tyr mutant enzyme was less than 10 min at 80°C, while that of the wild-type enzyme was about 90 min. Moreover, the residual activity of Cys-211→Ser/Tyr enzyme was substantially decreased by 8 M urea; and it lost all catalytic activity in 40% ethanol. These results show that the substitution of Cys with any amino acid residues at position 211 seems to affect the conformational stability of the chitosanase.

Original languageEnglish
Pages (from-to)173-180
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume56
Issue number1-2
DOIs
Publication statusPublished - 2001 Jul 30

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chitosanase
Mutagenesis
Essential Amino Acids
Site-Directed Mutagenesis
Amino acids
Catalyst activity
Enzymes
Amino Acids
Bacilli
Carboxylic Acids
Catalysis
Carboxylic acids
Kinetic parameters
Urea
Bacillus
Half-Life
Ethanol
Substitution reactions
Hot Temperature

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Identification of essential amino acid residues for catalytic activity and thermostability of novel chitosanase by site-directed mutagenesis. / Yoon, H. G.; Kim, H. Y.; Lim, Young Hee; Kim, H. K.; Shin, D. H.; Hong, B. S.; Cho, H. Y.

In: Applied Microbiology and Biotechnology, Vol. 56, No. 1-2, 30.07.2001, p. 173-180.

Research output: Contribution to journalArticle

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AU - Hong, B. S.

AU - Cho, H. Y.

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