Identification of expressed sequence tags of watermelon (Citrullus lanatus) leaf at the vegetative stage

S. Ok, Y. S. Chung, B. Y. Um, M. S. Park, J. M. Bae, S. J. Lee, J. S. Shin

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A cDNA library was constructed using mRNA prepared from leaves of watermelon [Citrullus lanatus (Thunb.) Matsum and Nakai] at the vegetative stage. Randomly selected cDNA clones were sequenced in order to identify potentially informative genes. Database comparisons indicated that out of the 704 watermelon cDNA clones, 399 clones (56.7%) revealed a high degree of sequence similarity to genes from other organisms. These expressed sequence tag clones were divided into ten categories depending upon gene function. Since this kind of experiment has not previously been carried out in this genome, random nucleotide sequencing of these cDNAs could contribute considerable information concerning the novel genes in this organism.

Original languageEnglish
Pages (from-to)932-937
Number of pages6
JournalPlant Cell Reports
Volume19
Issue number9
DOIs
Publication statusPublished - 2000

Keywords

  • CDNA sequence
  • Citrullus lanatus
  • Expressed sequence tags
  • Watermelon

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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