Identification of factors regulating Escherichia coli 2,3-butanediol production by continuous culture and metabolic flux analysis

Mingshou Lu, Soojin Lee, Borim Kim, Changhun Park, Min-Kyu Oh, Kyungmoon Park, Sang Yup Lee, Jinwon Lee

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia coli is often used for the production of organic compounds, the wild-type E. coli does not contain two essential genes in the 2,3-BDO biosynthesis pathway, and cannot ferment 2,3-BDO. Therefore, a 2,3-BDO biosynthesis mutant strain of Escherichia coli was constructed and cultured. To determine the optimum culture factors for 2,3-BDO production, experiments were conducted under different culture environments ranging from strongly acidic to neutral pH. The extracellular metabolite profiles were obtained using high-performance liquid chromatography (HPLC), and the intracellular metabolite profiles were analyzed by ultra-performance liquid chromatography and quadruple time-of-flight mass spectrometry (UPLC/ Q-TOF-MS). Metabolic flux analysis (MFA) was used to integrate these profiles. The metabolite profiles showed that 2,3-BDO production favors an acidic environment (pH 5), whereas cell mass favors a neutral environment. Furthermore, when the pH of the culture fell below 5, both the cell growth and 2,3-BDO production were inhibited.

Original languageEnglish
Pages (from-to)659-667
Number of pages9
JournalJournal of Microbiology and Biotechnology
Volume22
Issue number5
DOIs
Publication statusPublished - 2012 May 1

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Metabolic Flux Analysis
Escherichia coli
Essential Genes
2,3-butylene glycol
Liquid Chromatography
Mass Spectrometry
High Pressure Liquid Chromatography

Keywords

  • 2,3-butanediol fermentation
  • Continuous culture
  • Intracellular metabolic measurements
  • Metabolic flux analysis
  • pH influence

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Identification of factors regulating Escherichia coli 2,3-butanediol production by continuous culture and metabolic flux analysis. / Lu, Mingshou; Lee, Soojin; Kim, Borim; Park, Changhun; Oh, Min-Kyu; Park, Kyungmoon; Lee, Sang Yup; Lee, Jinwon.

In: Journal of Microbiology and Biotechnology, Vol. 22, No. 5, 01.05.2012, p. 659-667.

Research output: Contribution to journalArticle

Lu, Mingshou ; Lee, Soojin ; Kim, Borim ; Park, Changhun ; Oh, Min-Kyu ; Park, Kyungmoon ; Lee, Sang Yup ; Lee, Jinwon. / Identification of factors regulating Escherichia coli 2,3-butanediol production by continuous culture and metabolic flux analysis. In: Journal of Microbiology and Biotechnology. 2012 ; Vol. 22, No. 5. pp. 659-667.
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