Identification of factors regulating Escherichia coli 2,3-butanediol production by continuous culture and metabolic flux analysis

Mingshou Lu, Soojin Lee, Borim Kim, Changhun Park, Minkyu Oh, Kyungmoon Park, Sang Yup Lee, Jinwon Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

2,3-Butanediol (2,3-BDO) is an organic compound with a wide range of industrial applications. Although Escherichia coli is often used for the production of organic compounds, the wild-type E. coli does not contain two essential genes in the 2,3-BDO biosynthesis pathway, and cannot ferment 2,3-BDO. Therefore, a 2,3-BDO biosynthesis mutant strain of Escherichia coli was constructed and cultured. To determine the optimum culture factors for 2,3-BDO production, experiments were conducted under different culture environments ranging from strongly acidic to neutral pH. The extracellular metabolite profiles were obtained using high-performance liquid chromatography (HPLC), and the intracellular metabolite profiles were analyzed by ultra-performance liquid chromatography and quadruple time-of-flight mass spectrometry (UPLC/ Q-TOF-MS). Metabolic flux analysis (MFA) was used to integrate these profiles. The metabolite profiles showed that 2,3-BDO production favors an acidic environment (pH 5), whereas cell mass favors a neutral environment. Furthermore, when the pH of the culture fell below 5, both the cell growth and 2,3-BDO production were inhibited.

Original languageEnglish
Pages (from-to)659-667
Number of pages9
JournalJournal of microbiology and biotechnology
Volume22
Issue number5
DOIs
Publication statusPublished - 2012 May

Keywords

  • 2,3-butanediol fermentation
  • Continuous culture
  • Intracellular metabolic measurements
  • Metabolic flux analysis
  • pH influence

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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