Identification of farnesyl pyrophosphate and N-arachidonylglycine as endogenous ligands for GPR92

Da Young Oh, Min Yoon Jung, Jin Moon Mi, Jong-Ik Hwang, Choe Han, Yeon Lee Ju, Il Kim Jae, Kim Sunoh, Rhim Hyewhon, K. O Dell David, J. Walker Michael, Heung Sik Na, Goo Lee Min, Bang Kwon Hyuk, Kim Kyungjin, Jae Young Seong

Research output: Contribution to journalArticle

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Abstract

A series of small compounds acting at the orphan G protein-coupled receptor GPR92 were screened using a signaling pathway-specific reporter assay system. Lipid-derived molecules including farnesyl pyrophosphate (FPP), N-arachidonylglycine (NAG), and lysophosphatidic acid were found to activate GPR92. FPP and lysophosphatidic acid were able to activate both G q/11- and Gs-mediated signaling pathways, whereas NAG activated only the Gq/11-mediated signaling pathway. Computer-simulated modeling combined with site-directed mutagenesis of GPR92 indicated that Thr97, Gly98, Phe101, and Arg267 of GPR92 are responsible for the interaction of GPR92 with FPP and NAG. Reverse transcription-PCR analysis revealed that GPR92 mRNA is highly expressed in the dorsal root ganglia (DRG) but faint in other brain regions. Peripheral tissues including, spleen, stomach, small intestine, and kidney also expressed GPR92 mRNA. Immunohistochemical analysis revealed that GPR92 is largely co-localized with TRPV1, a nonspecific cation channel that responds to noxious heat, in mouse and human DRG. FPP and NAG increased intracellular Ca2+ levels in cultured DRG neurons. These results suggest that FPP and NAG play a role in the sensory nervous system through activation of GPR92.

Original languageEnglish
Pages (from-to)21054-21064
Number of pages11
JournalJournal of Biological Chemistry
Volume283
Issue number30
DOIs
Publication statusPublished - 2008 Jul 25

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Ligands
Spinal Ganglia
Mutagenesis
Messenger RNA
Neurology
Transcription
G-Protein-Coupled Receptors
Site-Directed Mutagenesis
Nervous System
Reverse Transcription
Neurons
Small Intestine
Cations
Assays
Brain
Stomach
Spleen
Hot Temperature
Chemical activation
farnesyl pyrophosphate

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Identification of farnesyl pyrophosphate and N-arachidonylglycine as endogenous ligands for GPR92. / Oh, Da Young; Jung, Min Yoon; Mi, Jin Moon; Hwang, Jong-Ik; Han, Choe; Ju, Yeon Lee; Jae, Il Kim; Sunoh, Kim; Hyewhon, Rhim; David, K. O Dell; Michael, J. Walker; Na, Heung Sik; Min, Goo Lee; Hyuk, Bang Kwon; Kyungjin, Kim; Seong, Jae Young.

In: Journal of Biological Chemistry, Vol. 283, No. 30, 25.07.2008, p. 21054-21064.

Research output: Contribution to journalArticle

Oh, DY, Jung, MY, Mi, JM, Hwang, J-I, Han, C, Ju, YL, Jae, IK, Sunoh, K, Hyewhon, R, David, KOD, Michael, JW, Na, HS, Min, GL, Hyuk, BK, Kyungjin, K & Seong, JY 2008, 'Identification of farnesyl pyrophosphate and N-arachidonylglycine as endogenous ligands for GPR92', Journal of Biological Chemistry, vol. 283, no. 30, pp. 21054-21064. https://doi.org/10.1074/jbc.M708908200
Oh, Da Young ; Jung, Min Yoon ; Mi, Jin Moon ; Hwang, Jong-Ik ; Han, Choe ; Ju, Yeon Lee ; Jae, Il Kim ; Sunoh, Kim ; Hyewhon, Rhim ; David, K. O Dell ; Michael, J. Walker ; Na, Heung Sik ; Min, Goo Lee ; Hyuk, Bang Kwon ; Kyungjin, Kim ; Seong, Jae Young. / Identification of farnesyl pyrophosphate and N-arachidonylglycine as endogenous ligands for GPR92. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 30. pp. 21054-21064.
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T1 - Identification of farnesyl pyrophosphate and N-arachidonylglycine as endogenous ligands for GPR92

AU - Oh, Da Young

AU - Jung, Min Yoon

AU - Mi, Jin Moon

AU - Hwang, Jong-Ik

AU - Han, Choe

AU - Ju, Yeon Lee

AU - Jae, Il Kim

AU - Sunoh, Kim

AU - Hyewhon, Rhim

AU - David, K. O Dell

AU - Michael, J. Walker

AU - Na, Heung Sik

AU - Min, Goo Lee

AU - Hyuk, Bang Kwon

AU - Kyungjin, Kim

AU - Seong, Jae Young

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