Identification of genes associated with fumonisin biosynthesis in Fusarium verticillioides via proteomics and quantitative real-time PCR

Yoon E. Choi, Won Bo Shim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In this study, we used functional genomic strategies, proteomics and quantitative real-time (qRT)-PCR, to advance our understanding of genes associated with fumonisin production in the fungus Fusarium verticillioides. Earlier studies have demonstrated that deletion of the FCC1 gene, which encodes a C-type cyclin, leads to a drastic reduction in fumonisin production and conidiation in the mutant strain (FT536). The premise of our research was that comparative analysis of F. verticillioides wild-type and FT536 proteomes will reveal putative proteins, and ultimately corresponding genes, that are important for fumonisin biosynthesis. We isolated proteins that were significantly upregulated in either the wild type or FT536 via two-dimensional polyacrylamide gel electrophoresis, and subsequently obtained sequences by mass spectrometry. Homologs of identified proteins, e.g., carboxypeptidase, laccase, and nitrogen metabolite repression protein, are known to have functions involved in fungal secondary metabolism and development. We also identified gene sequences corresponding to the selected proteins and investigated their transcriptional profiles via quantitative real-time (qRT)-PCR in order to identify genes that show concomitant expression patterns during fumonisin biosynthesis. These genes can be selected as targets for functional analysis to further verify their roles in FB1 biosynthesis.

Original languageEnglish
Pages (from-to)648-657
Number of pages10
JournalJournal of microbiology and biotechnology
Volume18
Issue number4
Publication statusPublished - 2008 Apr 28
Externally publishedYes

Keywords

  • Fumonisin regulation
  • Fungal development
  • Gene discovery
  • Proteomics
  • Quantitative real-time PCR

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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