Identification of genes induced in wound-treated wild rice (Oryza minuta)

Sung Ki Cho, Ji Ung Jeung, Kyung Ho Kang, Kyu Suk Shim, Kwang Wook Jung, Min Kyoung You, Sung Han Ok, Young Soo Chung, Heung Gu Hwang, Hae Chune Choi, Huhn Pal Moon, Jeong Sheop Shin

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6 Citations (Scopus)

Abstract

A subtracted library was constructed from wound-treated wild rice (Oryza minuta) by suppression subtractive hybridization (SSH) in combination with mirror orientation selection (MOS). To distinguish between differentially expressed transcripts and false positive clones, DNA chips containing 960 random clones were applied as a form of reverse Northern screening. Based on the signal intensities and expression ratios obtained from experiments performed in triplicate, 371 clones were selected. ESTs produced from the subtracted library showed 63.2% redundancy, and 72% of all clones could be matched to the GenBank nonredundant database. Functional categorization placed the identified enriched genes in categories of subcellular localization, metabolism, cell rescue and defense, and transcription. These EST-related resistance mechanisms could be used in investigations into the defense mechanisms of wild species, and to provide new routes to improving the germplasm of cultivated rice.

Original languageEnglish
Pages (from-to)230-236
Number of pages7
JournalMolecules and Cells
Volume17
Issue number2
Publication statusPublished - 2004 Apr 30

Keywords

  • EST
  • Microarray
  • Oryza minuta
  • Suppression subtractive hybridization
  • Wild rice
  • Wound

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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    Cho, S. K., Jeung, J. U., Kang, K. H., Shim, K. S., Jung, K. W., You, M. K., Ok, S. H., Chung, Y. S., Hwang, H. G., Choi, H. C., Moon, H. P., & Shin, J. S. (2004). Identification of genes induced in wound-treated wild rice (Oryza minuta). Molecules and Cells, 17(2), 230-236.