Identification of genes induced in wound-treated wild rice (Oryza minuta)

Sung Ki Cho, Ji Ung Jeung, Kyung Ho Kang, Kyu Suk Shim, Kwang Wook Jung, Min Kyoung You, Sung Han Ok, Young Soo Chung, Heung Gu Hwang, Hae Chune Choi, Huhn Pal Moon, Jeong Sheop Shin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A subtracted library was constructed from wound-treated wild rice (Oryza minuta) by suppression subtractive hybridization (SSH) in combination with mirror orientation selection (MOS). To distinguish between differentially expressed transcripts and false positive clones, DNA chips containing 960 random clones were applied as a form of reverse Northern screening. Based on the signal intensities and expression ratios obtained from experiments performed in triplicate, 371 clones were selected. ESTs produced from the subtracted library showed 63.2% redundancy, and 72% of all clones could be matched to the GenBank nonredundant database. Functional categorization placed the identified enriched genes in categories of subcellular localization, metabolism, cell rescue and defense, and transcription. These EST-related resistance mechanisms could be used in investigations into the defense mechanisms of wild species, and to provide new routes to improving the germplasm of cultivated rice.

Original languageEnglish
Pages (from-to)230-236
Number of pages7
JournalMolecules and Cells
Volume17
Issue number2
Publication statusPublished - 2004 Apr 30

Fingerprint

Clone Cells
Expressed Sequence Tags
Wounds and Injuries
Genes
Nucleic Acid Databases
Oligonucleotide Array Sequence Analysis
Databases
Oryza

Keywords

  • EST
  • Microarray
  • Oryza minuta
  • Suppression subtractive hybridization
  • Wild rice
  • Wound

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Cho, S. K., Jeung, J. U., Kang, K. H., Shim, K. S., Jung, K. W., You, M. K., ... Shin, J. S. (2004). Identification of genes induced in wound-treated wild rice (Oryza minuta). Molecules and Cells, 17(2), 230-236.

Identification of genes induced in wound-treated wild rice (Oryza minuta). / Cho, Sung Ki; Jeung, Ji Ung; Kang, Kyung Ho; Shim, Kyu Suk; Jung, Kwang Wook; You, Min Kyoung; Ok, Sung Han; Chung, Young Soo; Hwang, Heung Gu; Choi, Hae Chune; Moon, Huhn Pal; Shin, Jeong Sheop.

In: Molecules and Cells, Vol. 17, No. 2, 30.04.2004, p. 230-236.

Research output: Contribution to journalArticle

Cho, SK, Jeung, JU, Kang, KH, Shim, KS, Jung, KW, You, MK, Ok, SH, Chung, YS, Hwang, HG, Choi, HC, Moon, HP & Shin, JS 2004, 'Identification of genes induced in wound-treated wild rice (Oryza minuta)', Molecules and Cells, vol. 17, no. 2, pp. 230-236.
Cho SK, Jeung JU, Kang KH, Shim KS, Jung KW, You MK et al. Identification of genes induced in wound-treated wild rice (Oryza minuta). Molecules and Cells. 2004 Apr 30;17(2):230-236.
Cho, Sung Ki ; Jeung, Ji Ung ; Kang, Kyung Ho ; Shim, Kyu Suk ; Jung, Kwang Wook ; You, Min Kyoung ; Ok, Sung Han ; Chung, Young Soo ; Hwang, Heung Gu ; Choi, Hae Chune ; Moon, Huhn Pal ; Shin, Jeong Sheop. / Identification of genes induced in wound-treated wild rice (Oryza minuta). In: Molecules and Cells. 2004 ; Vol. 17, No. 2. pp. 230-236.
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