Identification of genes possibly related to storage root induction in sweetpotato

Min Kyoung You, Cheol Goo Hur, Young Sup Ahn, Mi Chung Suh, Byeong Choon Jeong, Jeong Sheop Shin, Jung Myung Bae

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

To identify genes related to initiation of storage root development in sweetpotato, a cDNA library was constructed with early stage storage roots (0.3-1 cm in diameter). Single-pass sequences of the 5′ ends of 2859 sweetpotato cDNA clones were assembled into 483 clusters and 442 singletons. Comparison of sweetpotato expressed sequence tags (ESTs) to nodulation/tumorigenesis-related sequence databases (nodule-, tumor-, potato tuber- and development-related sequences) revealed that homologs of 39 sweetpotato EST sequences potentially involved in gene regulation, signal transduction and development were present in at least one of the nodulation/tumorigenesis-related sequence databases. Northern blot analyses of these 39 sequences identified 22 differentially expressed genes in early stage storage root and fibrous root. These differentially expressed genes will be potential candidates for research to elucidate the molecular processes related to sweetpotato storage root induction.

Original languageEnglish
Pages (from-to)101-105
Number of pages5
JournalFEBS Letters
Volume536
Issue number1-3
DOIs
Publication statusPublished - 2003 Feb 11

Fingerprint

Genes
Expressed Sequence Tags
Signal transduction
Carcinogenesis
Databases
Gene Library
Gene expression
Tumors
Sequence Homology
Solanum tuberosum
Complementary DNA
Northern Blotting
Sequence Analysis
Signal Transduction
Clone Cells
Research
Neoplasms

Keywords

  • Comparative analysis
  • Expressed sequence tag
  • Ipomoea batatas
  • Storage root
  • Storage root induction
  • Sweetpotato

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

You, M. K., Hur, C. G., Ahn, Y. S., Suh, M. C., Jeong, B. C., Shin, J. S., & Bae, J. M. (2003). Identification of genes possibly related to storage root induction in sweetpotato. FEBS Letters, 536(1-3), 101-105. https://doi.org/10.1016/S0014-5793(03)00035-8

Identification of genes possibly related to storage root induction in sweetpotato. / You, Min Kyoung; Hur, Cheol Goo; Ahn, Young Sup; Suh, Mi Chung; Jeong, Byeong Choon; Shin, Jeong Sheop; Bae, Jung Myung.

In: FEBS Letters, Vol. 536, No. 1-3, 11.02.2003, p. 101-105.

Research output: Contribution to journalArticle

You, MK, Hur, CG, Ahn, YS, Suh, MC, Jeong, BC, Shin, JS & Bae, JM 2003, 'Identification of genes possibly related to storage root induction in sweetpotato', FEBS Letters, vol. 536, no. 1-3, pp. 101-105. https://doi.org/10.1016/S0014-5793(03)00035-8
You, Min Kyoung ; Hur, Cheol Goo ; Ahn, Young Sup ; Suh, Mi Chung ; Jeong, Byeong Choon ; Shin, Jeong Sheop ; Bae, Jung Myung. / Identification of genes possibly related to storage root induction in sweetpotato. In: FEBS Letters. 2003 ; Vol. 536, No. 1-3. pp. 101-105.
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