Abstract
This paper describes the differentiation between native Korean cattle (Hanwoo) and Holsteins or imported cattle using the real-time polymerase chain reaction (PCR) by targeting the sequence of the melanocortin 1 receptor (MC1R) gene. A rapid and accurate method was developed to identify Hanwoo by genotyping the DNA extracted from 295 commercial beef samples (obtained from 5 provinces in South Korea) labeled as Hanwoo beef. The results of real-time PCR assays for the proportions of Hanwoo were 84, 85.7, 95, 91.4, and 90% in the areas of Seoul, Joongbu, Youngnam, Honam, and Chungcheong, respectively. Thus, the beef samples from 295 butcher shops, which asserted to only sell Hanwoo, showed that 259 of 295 samples were of the Hanwoo beef gene type (T-type) and 36 of 295 samples were Holsteins of imported dairy cattle gene types (C-type or C/T type). In conclusion, the proportion of Hanwoo beef was 87.8% and the proportion of Holstein or imported dairy cattle meat was 12.2% (C-type: 9.8%, C/T-type: 2.4%). Generally, most consumers can not differentiate imported meat from Hanwoo beef. Therefore, Hanwoo beef and imported dairy cattle meat that is sold in butcher shops should have mandatory identification by using MC1R genotyping based on real-time PCR.
| Original language | English |
|---|---|
| Pages (from-to) | 668-672 |
| Number of pages | 5 |
| Journal | Korean Journal for Food Science of Animal Resources |
| Volume | 29 |
| Issue number | 6 |
| Publication status | Published - 2009 Dec 1 |
| Externally published | Yes |
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Keywords
- Hanwoo (korean native cattle)
- MC1R
- Real time pcr
ASJC Scopus subject areas
- Food Science
- Animal Science and Zoology
Cite this
Identification of Hanwoo (native Korean cattle breed) beef by real-time PCR using the MC1R gene in 5 provinces of South Korea. / Park, Jung Min; Shin, Jin Ho; Lee, Dan Won; Song, Jae Chul; Suh, Hyung Joo; Chang, Un Jae; Kim, Jin Man.
In: Korean Journal for Food Science of Animal Resources, Vol. 29, No. 6, 01.12.2009, p. 668-672.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of Hanwoo (native Korean cattle breed) beef by real-time PCR using the MC1R gene in 5 provinces of South Korea
AU - Park, Jung Min
AU - Shin, Jin Ho
AU - Lee, Dan Won
AU - Song, Jae Chul
AU - Suh, Hyung Joo
AU - Chang, Un Jae
AU - Kim, Jin Man
PY - 2009/12/1
Y1 - 2009/12/1
N2 - This paper describes the differentiation between native Korean cattle (Hanwoo) and Holsteins or imported cattle using the real-time polymerase chain reaction (PCR) by targeting the sequence of the melanocortin 1 receptor (MC1R) gene. A rapid and accurate method was developed to identify Hanwoo by genotyping the DNA extracted from 295 commercial beef samples (obtained from 5 provinces in South Korea) labeled as Hanwoo beef. The results of real-time PCR assays for the proportions of Hanwoo were 84, 85.7, 95, 91.4, and 90% in the areas of Seoul, Joongbu, Youngnam, Honam, and Chungcheong, respectively. Thus, the beef samples from 295 butcher shops, which asserted to only sell Hanwoo, showed that 259 of 295 samples were of the Hanwoo beef gene type (T-type) and 36 of 295 samples were Holsteins of imported dairy cattle gene types (C-type or C/T type). In conclusion, the proportion of Hanwoo beef was 87.8% and the proportion of Holstein or imported dairy cattle meat was 12.2% (C-type: 9.8%, C/T-type: 2.4%). Generally, most consumers can not differentiate imported meat from Hanwoo beef. Therefore, Hanwoo beef and imported dairy cattle meat that is sold in butcher shops should have mandatory identification by using MC1R genotyping based on real-time PCR.
AB - This paper describes the differentiation between native Korean cattle (Hanwoo) and Holsteins or imported cattle using the real-time polymerase chain reaction (PCR) by targeting the sequence of the melanocortin 1 receptor (MC1R) gene. A rapid and accurate method was developed to identify Hanwoo by genotyping the DNA extracted from 295 commercial beef samples (obtained from 5 provinces in South Korea) labeled as Hanwoo beef. The results of real-time PCR assays for the proportions of Hanwoo were 84, 85.7, 95, 91.4, and 90% in the areas of Seoul, Joongbu, Youngnam, Honam, and Chungcheong, respectively. Thus, the beef samples from 295 butcher shops, which asserted to only sell Hanwoo, showed that 259 of 295 samples were of the Hanwoo beef gene type (T-type) and 36 of 295 samples were Holsteins of imported dairy cattle gene types (C-type or C/T type). In conclusion, the proportion of Hanwoo beef was 87.8% and the proportion of Holstein or imported dairy cattle meat was 12.2% (C-type: 9.8%, C/T-type: 2.4%). Generally, most consumers can not differentiate imported meat from Hanwoo beef. Therefore, Hanwoo beef and imported dairy cattle meat that is sold in butcher shops should have mandatory identification by using MC1R genotyping based on real-time PCR.
KW - Hanwoo (korean native cattle)
KW - MC1R
KW - Real time pcr
UR - http://www.scopus.com/inward/record.url?scp=76649121495&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=76649121495&partnerID=8YFLogxK
M3 - Article
VL - 29
SP - 668
EP - 672
JO - Korean Journal for Food Science of Animal Resources
JF - Korean Journal for Food Science of Animal Resources
SN - 1225-8563
IS - 6
ER -