Identification of immunogenic antigen candidate for Chlamydophila pneumoniae diagnosis

Sung Ha Park, Su Jin Kwon, Sun Jin Lee, Young Chang Kim, Kwang Yeon Hwang, Yeon Ho Kang, Kwang Jun Lee

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Chlamydophila pneumoniae is a Gram-negative intracellular obligate human pathogen and accounts for 5-10% of cases of community-acquired pneumonia. However, isolating and culturing this pathogen is difficult, so there have been several studies searching for new biomarkers for its diagnosis. In this study, we obtained immunogenic proteins of C. pneumoniae KNIH-1 for diagnosis using immunoproteomics. C. pneumoniae infection sera were selected for the highest index value of C. pneumoniaespecific IgG using microimmunofluorescence (MIF). The detected protein spots in common from C. pneumoniae infection sera using proteome analysis were identified as Omp11, type III secretion system ATPase, and PmpG by LC-MS/MS and MS databases. They were selected as candidate antigens. In addition, using in silico prediction we also identified proteins encoded by Omp11, PmpG and IncA as antigens. And then, IncA acts as an effector by a type III secretion system ATPase, as identified by mass spectrometry, and was selected as a candidate antigen. Thus, we predict proteins encoded by Omp11, the PmpG family and by IncA as candidate diagnostic immunogens.

Original languageEnglish
Pages (from-to)2933-2943
Number of pages11
JournalJournal of Proteome Research
Volume8
Issue number6
DOIs
Publication statusPublished - 2009 Jun 5

Fingerprint

Chlamydophila pneumoniae
Pathogens
Antigens
Adenosine Triphosphatases
Pneumonia
Proteins
Biomarkers
Proteome
Protein C
Infection
Serum
Computer Simulation
Mass spectrometry
Mass Spectrometry
Immunoglobulin G
Databases
Type III Secretion Systems

Keywords

  • Chlamydophila pneumoniae
  • Immunogenic antigen
  • Immunoproteomics
  • IncA
  • Mass spectrometry
  • Omp11
  • PmpG
  • Tandem liquid chromatography
  • Two-dimensional gel electrophoresis

ASJC Scopus subject areas

  • Biochemistry
  • Chemistry(all)

Cite this

Identification of immunogenic antigen candidate for Chlamydophila pneumoniae diagnosis. / Park, Sung Ha; Kwon, Su Jin; Lee, Sun Jin; Kim, Young Chang; Hwang, Kwang Yeon; Kang, Yeon Ho; Lee, Kwang Jun.

In: Journal of Proteome Research, Vol. 8, No. 6, 05.06.2009, p. 2933-2943.

Research output: Contribution to journalArticle

Park, Sung Ha ; Kwon, Su Jin ; Lee, Sun Jin ; Kim, Young Chang ; Hwang, Kwang Yeon ; Kang, Yeon Ho ; Lee, Kwang Jun. / Identification of immunogenic antigen candidate for Chlamydophila pneumoniae diagnosis. In: Journal of Proteome Research. 2009 ; Vol. 8, No. 6. pp. 2933-2943.
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