Identification of lignin-deficient Brachypodium distachyon (L.) Beauv. mutants induced by gamma radiation

Man Bo Lee, Jae Yoon Kim, Yong Weon Seo

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1 Citation (Scopus)

Abstract

BACKGROUND: Brachypodium distachyon (L.) Beauv. is a monocotyledonous model plant that has been studied to understand a range of biological phenomena for lignocellulosic bioethanol feedstocks and other cereal crops. The lignin makes its cell walls recalcitrant to saccharification, constituting the main barrier to lignocellulosic bioethanol production. In this study, lignin-deficient mutants of B. distachyon induced by chronic radiation were selected and the effects of the mutants on fermentable glucose production were identified. RESULTS: Brachypodium distachyon M2 mutants induced by chronically irradiated gamma radiation were screened by the Wiesner test. Lignin-deficient M2 mutants were further confirmed in subsequent M3 and M4 generations by determining acetyl bromide-soluble lignin. The lignin content was significantly reduced in mutant plants 135-2 (by 7.99%), 142-3 (by 13.8%) and 406-1 (by 8.13%) compared with the wild type. Moreover, fermentable glucose was significantly higher in 135-2 (by 23.91%) and 142-3 (by 36.72%) than in the wild type after 72 h of enzymatic hydrolysis. CONCLUSION: Three lignin-deficient B. distachyon mutants induced by chronically irradiated gamma radiation were obtained. This study will provide fundamental understanding of the B. distachyon cell wall and could contribute to increases in bioethanol production using bioenergy crops.

Original languageEnglish
JournalJournal of the Science of Food and Agriculture
DOIs
Publication statusAccepted/In press - 2016

Fingerprint

Brachypodium
Brachypodium distachyon
Lignin
Gamma Rays
gamma radiation
lignin
mutants
ethanol production
Cell Wall
cell walls
Biological Phenomena
Glucose
bioethanol
glucose
saccharification
energy crops
enzymatic hydrolysis
grain crops
feedstocks
bromides

Keywords

  • Brachypodium distachyon
  • Enzyme hydrolysis
  • Gamma radiation
  • Lignin
  • Lignocellulosic bioethanol
  • Mutation

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Agronomy and Crop Science
  • Nutrition and Dietetics

Cite this

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title = "Identification of lignin-deficient Brachypodium distachyon (L.) Beauv. mutants induced by gamma radiation",
abstract = "BACKGROUND: Brachypodium distachyon (L.) Beauv. is a monocotyledonous model plant that has been studied to understand a range of biological phenomena for lignocellulosic bioethanol feedstocks and other cereal crops. The lignin makes its cell walls recalcitrant to saccharification, constituting the main barrier to lignocellulosic bioethanol production. In this study, lignin-deficient mutants of B. distachyon induced by chronic radiation were selected and the effects of the mutants on fermentable glucose production were identified. RESULTS: Brachypodium distachyon M2 mutants induced by chronically irradiated gamma radiation were screened by the Wiesner test. Lignin-deficient M2 mutants were further confirmed in subsequent M3 and M4 generations by determining acetyl bromide-soluble lignin. The lignin content was significantly reduced in mutant plants 135-2 (by 7.99{\%}), 142-3 (by 13.8{\%}) and 406-1 (by 8.13{\%}) compared with the wild type. Moreover, fermentable glucose was significantly higher in 135-2 (by 23.91{\%}) and 142-3 (by 36.72{\%}) than in the wild type after 72 h of enzymatic hydrolysis. CONCLUSION: Three lignin-deficient B. distachyon mutants induced by chronically irradiated gamma radiation were obtained. This study will provide fundamental understanding of the B. distachyon cell wall and could contribute to increases in bioethanol production using bioenergy crops.",
keywords = "Brachypodium distachyon, Enzyme hydrolysis, Gamma radiation, Lignin, Lignocellulosic bioethanol, Mutation",
author = "Lee, {Man Bo} and Kim, {Jae Yoon} and Seo, {Yong Weon}",
year = "2016",
doi = "10.1002/jsfa.8024",
language = "English",
journal = "Journal of the Science of Food and Agriculture",
issn = "0022-5142",
publisher = "John Wiley and Sons Ltd",

}

TY - JOUR

T1 - Identification of lignin-deficient Brachypodium distachyon (L.) Beauv. mutants induced by gamma radiation

AU - Lee, Man Bo

AU - Kim, Jae Yoon

AU - Seo, Yong Weon

PY - 2016

Y1 - 2016

N2 - BACKGROUND: Brachypodium distachyon (L.) Beauv. is a monocotyledonous model plant that has been studied to understand a range of biological phenomena for lignocellulosic bioethanol feedstocks and other cereal crops. The lignin makes its cell walls recalcitrant to saccharification, constituting the main barrier to lignocellulosic bioethanol production. In this study, lignin-deficient mutants of B. distachyon induced by chronic radiation were selected and the effects of the mutants on fermentable glucose production were identified. RESULTS: Brachypodium distachyon M2 mutants induced by chronically irradiated gamma radiation were screened by the Wiesner test. Lignin-deficient M2 mutants were further confirmed in subsequent M3 and M4 generations by determining acetyl bromide-soluble lignin. The lignin content was significantly reduced in mutant plants 135-2 (by 7.99%), 142-3 (by 13.8%) and 406-1 (by 8.13%) compared with the wild type. Moreover, fermentable glucose was significantly higher in 135-2 (by 23.91%) and 142-3 (by 36.72%) than in the wild type after 72 h of enzymatic hydrolysis. CONCLUSION: Three lignin-deficient B. distachyon mutants induced by chronically irradiated gamma radiation were obtained. This study will provide fundamental understanding of the B. distachyon cell wall and could contribute to increases in bioethanol production using bioenergy crops.

AB - BACKGROUND: Brachypodium distachyon (L.) Beauv. is a monocotyledonous model plant that has been studied to understand a range of biological phenomena for lignocellulosic bioethanol feedstocks and other cereal crops. The lignin makes its cell walls recalcitrant to saccharification, constituting the main barrier to lignocellulosic bioethanol production. In this study, lignin-deficient mutants of B. distachyon induced by chronic radiation were selected and the effects of the mutants on fermentable glucose production were identified. RESULTS: Brachypodium distachyon M2 mutants induced by chronically irradiated gamma radiation were screened by the Wiesner test. Lignin-deficient M2 mutants were further confirmed in subsequent M3 and M4 generations by determining acetyl bromide-soluble lignin. The lignin content was significantly reduced in mutant plants 135-2 (by 7.99%), 142-3 (by 13.8%) and 406-1 (by 8.13%) compared with the wild type. Moreover, fermentable glucose was significantly higher in 135-2 (by 23.91%) and 142-3 (by 36.72%) than in the wild type after 72 h of enzymatic hydrolysis. CONCLUSION: Three lignin-deficient B. distachyon mutants induced by chronically irradiated gamma radiation were obtained. This study will provide fundamental understanding of the B. distachyon cell wall and could contribute to increases in bioethanol production using bioenergy crops.

KW - Brachypodium distachyon

KW - Enzyme hydrolysis

KW - Gamma radiation

KW - Lignin

KW - Lignocellulosic bioethanol

KW - Mutation

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