Abstract
Objective: A low pH microenvironment is a characteristic feature of inflammation loci and affects the functions of immune cells. In this study, we investigated the effect of extracellular acidification on macrophage gene expression. Methods: RAW264.7 macrophages were incubated in neutral (pH 7.4) or acidic (pH 6.8) medium for 4 h. Global mRNA expression levels were determined using Affymetrix genechips. Results: The mRNA expressions of 353 macrophage genes were significantly modified after incubation in acidic medium; 193 were up-regulated and 160 down-regulated. Differentially regulated genes were grouped into 13 classes based on the functions of the corresponding protein products. Pathway analysis revealed that differentially expressed genes are enriched in pathways related to inflammation and immune responses. Quantitative real-time PCR analysis confirmed that the expressions of CXCL10, CXCL14, IL-18, IL-4RA, ABCA1, CCL4, IL-7R, CXCR4, TLR7, and CCL3 mRNAs were regulated by extracellular acidification. Conclusion: The results of this study provide insights into the effects of acidic extracellular environments on macrophage gene expression.
| Original language | English |
|---|---|
| Pages (from-to) | 399-406 |
| Number of pages | 8 |
| Journal | Inflammation Research |
| Volume | 62 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2013 Apr 1 |
| Externally published | Yes |
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Keywords
- Extracellular acidification
- Gene expression
- Macrophages
- Microarray
ASJC Scopus subject areas
- Pharmacology
- Immunology
Cite this
Identification of macrophage genes responsive to extracellular acidification. / Park, Seung Yoon; Kim, In-San.
In: Inflammation Research, Vol. 62, No. 4, 01.04.2013, p. 399-406.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Identification of macrophage genes responsive to extracellular acidification
AU - Park, Seung Yoon
AU - Kim, In-San
PY - 2013/4/1
Y1 - 2013/4/1
N2 - Objective: A low pH microenvironment is a characteristic feature of inflammation loci and affects the functions of immune cells. In this study, we investigated the effect of extracellular acidification on macrophage gene expression. Methods: RAW264.7 macrophages were incubated in neutral (pH 7.4) or acidic (pH 6.8) medium for 4 h. Global mRNA expression levels were determined using Affymetrix genechips. Results: The mRNA expressions of 353 macrophage genes were significantly modified after incubation in acidic medium; 193 were up-regulated and 160 down-regulated. Differentially regulated genes were grouped into 13 classes based on the functions of the corresponding protein products. Pathway analysis revealed that differentially expressed genes are enriched in pathways related to inflammation and immune responses. Quantitative real-time PCR analysis confirmed that the expressions of CXCL10, CXCL14, IL-18, IL-4RA, ABCA1, CCL4, IL-7R, CXCR4, TLR7, and CCL3 mRNAs were regulated by extracellular acidification. Conclusion: The results of this study provide insights into the effects of acidic extracellular environments on macrophage gene expression.
AB - Objective: A low pH microenvironment is a characteristic feature of inflammation loci and affects the functions of immune cells. In this study, we investigated the effect of extracellular acidification on macrophage gene expression. Methods: RAW264.7 macrophages were incubated in neutral (pH 7.4) or acidic (pH 6.8) medium for 4 h. Global mRNA expression levels were determined using Affymetrix genechips. Results: The mRNA expressions of 353 macrophage genes were significantly modified after incubation in acidic medium; 193 were up-regulated and 160 down-regulated. Differentially regulated genes were grouped into 13 classes based on the functions of the corresponding protein products. Pathway analysis revealed that differentially expressed genes are enriched in pathways related to inflammation and immune responses. Quantitative real-time PCR analysis confirmed that the expressions of CXCL10, CXCL14, IL-18, IL-4RA, ABCA1, CCL4, IL-7R, CXCR4, TLR7, and CCL3 mRNAs were regulated by extracellular acidification. Conclusion: The results of this study provide insights into the effects of acidic extracellular environments on macrophage gene expression.
KW - Extracellular acidification
KW - Gene expression
KW - Macrophages
KW - Microarray
UR - http://www.scopus.com/inward/record.url?scp=84876694486&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84876694486&partnerID=8YFLogxK
U2 - 10.1007/s00011-013-0591-6
DO - 10.1007/s00011-013-0591-6
M3 - Article
C2 - 23417272
AN - SCOPUS:84876694486
VL - 62
SP - 399
EP - 406
JO - Inflammation Research
JF - Inflammation Research
SN - 1023-3830
IS - 4
ER -