Identification of molecular markers distinguishing adult neural stem cells in the subventricular and subcallosal zones

Joo Yeon Kim, Mohammed R. Shaker, Ju Hyun Lee, Boram Lee, Hyun Kim, Woong Sun

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Neural stem cells (NSCs) in the adult subventricular zone (SVZ) are regionally specified and have distinct molecular gene expression signatures. Recently, we identified the subcallosal zone (SCZ) as a novel brain region where adult NSCs maintain and spontaneously produce neuroblasts. In an attempt to isolate genes specifically expressed in the SCZ or SVZ, microarray analyses of their differentially expressing transcripts were done. The comparison between neurospheres generated from SVZ and SCZ revealed differential expression >1.5-fold in two groups in only 83 genes, representing <0.03% of the genes examined, suggesting that these two populations are largely similar. The differential expression patterns SCZ and SVZ genes were confirmed by RT-PCR and Western blots. The selective expressions of two genes (CRBP1, HMGA1) in SVZ-NSCs were further confirmed by immunohistochemistry. These molecular markers could be useful for further molecular and cellular characterization of NSCs.

Original languageEnglish
Pages (from-to)152-159
Number of pages8
JournalAnimal Cells and Systems
Volume21
Issue number3
DOIs
Publication statusPublished - 2017 May 17

Keywords

  • CRBP1
  • HMGA1
  • Subventricular zone
  • ZIC2
  • adult neural stem cell
  • microarray
  • subcallosal zone

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biochemistry, Genetics and Molecular Biology(all)

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