Identification of motifs for cell adhesion within the repeated domains of transforming growth factor-β-induced gene, βig-h3

Jung Eun Kim, Song Ja Kim, Byung Heon Lee, Rang Woon Park, Ki San Kim, In-San Kim

Research output: Contribution to journalArticle

214 Citations (Scopus)

Abstract

βig-h3 is a transforming growth factor-β-inducible cell adhesion molecule that has four characteristic homologous repeated domains. We made recombinant βig-h3 proteins, which were highly active in mediating human corneal epithelial (HCE) cell adhesion and spreading. The 2nd and the 4th repeated domains were sufficient to mediate HCE cell adhesion. A sequence analysis showed that aspartic acid (Asp) and isoleucine (Ile) of the 2nd and the 4th domains are highly conserved in many fasciclin 1 homologous (fas-1) domains. Substitution mutational study identified these two amino acids are essential for cell adhesion. Synthetic peptides containing Asp and Ile, NKDIL and EPDIM derived from the 2nd and the 4th domains, respectively, almost completely blocked cell adhesion mediated by not only wild type βig-h3 but also each of the 2nd and the 4th domains. These peptides alone were fully active in mediating cell adhesion. In addition, we demonstrated the functional receptor for βig-h3 is α3β1 integrin. These results, therefore, establish the essential motifs within the 2nd and the 4th domains of βig-h3, which interact with α3β1 integrin to mediate HCE cell adhesion to βig-h3 and suggest that other proteins containing Asp-Ile in their fas-1 domains could possibly function as cell adhesion molecules.

Original languageEnglish
Pages (from-to)30907-30915
Number of pages9
JournalJournal of Biological Chemistry
Volume275
Issue number40
DOIs
Publication statusPublished - 2000 Oct 6
Externally publishedYes

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Immunoglobulin Genes
Cell adhesion
Transforming Growth Factors
Cell Adhesion
Genes
Isoleucine
Aspartic Acid
Epithelial Cells
Cell Adhesion Molecules
Integrins
Histamine H3 Receptors
Peptides
Essential Amino Acids
Sequence Analysis
Proteins
Substitution reactions

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Identification of motifs for cell adhesion within the repeated domains of transforming growth factor-β-induced gene, βig-h3. / Kim, Jung Eun; Kim, Song Ja; Lee, Byung Heon; Park, Rang Woon; Kim, Ki San; Kim, In-San.

In: Journal of Biological Chemistry, Vol. 275, No. 40, 06.10.2000, p. 30907-30915.

Research output: Contribution to journalArticle

Kim, Jung Eun ; Kim, Song Ja ; Lee, Byung Heon ; Park, Rang Woon ; Kim, Ki San ; Kim, In-San. / Identification of motifs for cell adhesion within the repeated domains of transforming growth factor-β-induced gene, βig-h3. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 40. pp. 30907-30915.
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