Identification of mutations in OASA1 gene from a gamma-irradiated rice mutant population

Jae Buhm Chun, Bo Keun Ha, Duk Soo Jang, Mira Song, Kyung Jun Lee, Jin Baek Kim, Sang Hoon Kim, Si Yong Kang, Geung Joo Lee, Yong Weon Seo, Dong Sub Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

With 5 figures and 1 table One thousand three hundred and fifty homologous rice M 8 mutant lines resistant to 5-methyltryptophan, a tryptophan analogue, were selected from embryogenic calli irradiated with 30-120Gy of gamma rays. The mutant rice would be compromised in tryptophan homoeostasis in the cell if a negative feedback by anthranilate synthase (AS) in tryptophan biosynthesis was altered by gamma irradiation, consequently causing tryptophan over-accumulation. Therefore, the correlation between traits of over-accumulated tryptophan and the alteration of AS by mutagenesis including single-nucleotide polymorphisms (SNPs) would enable the visualization and selection of over-accumulated mutant lines from the rice mutant population. Targeting Induced Local Lesions IN Genome analysis with five fluorescent-labelled primer sets covering the OASA1 locus resulted in the selection of three rice mutant lines, MRVII-9, MRVII-33 and MRVII-37. The tryptophan contents in the seed storage protein of the three mutant lines were elevated when compared with the wild-type cv. 'Dongan'. Genomic DNA of OASA1 was re-sequenced and validated at the SNP and other polymorphism sites. Three mutant lines (MRVII-9, MRVII-33 and MRVII-37) not only contained more than five SNP sites in the total regions of OASA1 gene, but also contained SNPs in coding regions that resulted in amino acid changes such as E241G, A560G and V495F/S496F, respectively. The SNPs, which resulted in amino acid changes, may affect the enhanced tryptophan content in the seed storage protein. These different features could utilize the selection of mutant lines with over-accumulated tryptophan from large rice mutant populations.

Original languageEnglish
Pages (from-to)276-281
Number of pages6
JournalPlant Breeding
Volume131
Issue number2
DOIs
Publication statusPublished - 2012 Apr 1

Fingerprint

Tryptophan
tryptophan
mutation
rice
mutants
Mutation
Single Nucleotide Polymorphism
single nucleotide polymorphism
Population
Genes
Anthranilate Synthase
genes
Seed Storage Proteins
anthranilate synthase
seed storage proteins
gamma radiation
Amino Acids
Oryza
Gamma Rays
Bony Callus

Keywords

  • Anthranilate synthase
  • Mutation
  • Rice
  • Single-nucleotide polymorphisms
  • Targeting Induced Local Lesions IN Genome
  • Tryptophan

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science
  • Genetics

Cite this

Chun, J. B., Ha, B. K., Jang, D. S., Song, M., Lee, K. J., Kim, J. B., ... Kim, D. S. (2012). Identification of mutations in OASA1 gene from a gamma-irradiated rice mutant population. Plant Breeding, 131(2), 276-281. https://doi.org/10.1111/j.1439-0523.2011.01933.x

Identification of mutations in OASA1 gene from a gamma-irradiated rice mutant population. / Chun, Jae Buhm; Ha, Bo Keun; Jang, Duk Soo; Song, Mira; Lee, Kyung Jun; Kim, Jin Baek; Kim, Sang Hoon; Kang, Si Yong; Lee, Geung Joo; Seo, Yong Weon; Kim, Dong Sub.

In: Plant Breeding, Vol. 131, No. 2, 01.04.2012, p. 276-281.

Research output: Contribution to journalArticle

Chun, JB, Ha, BK, Jang, DS, Song, M, Lee, KJ, Kim, JB, Kim, SH, Kang, SY, Lee, GJ, Seo, YW & Kim, DS 2012, 'Identification of mutations in OASA1 gene from a gamma-irradiated rice mutant population', Plant Breeding, vol. 131, no. 2, pp. 276-281. https://doi.org/10.1111/j.1439-0523.2011.01933.x
Chun, Jae Buhm ; Ha, Bo Keun ; Jang, Duk Soo ; Song, Mira ; Lee, Kyung Jun ; Kim, Jin Baek ; Kim, Sang Hoon ; Kang, Si Yong ; Lee, Geung Joo ; Seo, Yong Weon ; Kim, Dong Sub. / Identification of mutations in OASA1 gene from a gamma-irradiated rice mutant population. In: Plant Breeding. 2012 ; Vol. 131, No. 2. pp. 276-281.
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AU - Lee, Kyung Jun

AU - Kim, Jin Baek

AU - Kim, Sang Hoon

AU - Kang, Si Yong

AU - Lee, Geung Joo

AU - Seo, Yong Weon

AU - Kim, Dong Sub

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N2 - With 5 figures and 1 table One thousand three hundred and fifty homologous rice M 8 mutant lines resistant to 5-methyltryptophan, a tryptophan analogue, were selected from embryogenic calli irradiated with 30-120Gy of gamma rays. The mutant rice would be compromised in tryptophan homoeostasis in the cell if a negative feedback by anthranilate synthase (AS) in tryptophan biosynthesis was altered by gamma irradiation, consequently causing tryptophan over-accumulation. Therefore, the correlation between traits of over-accumulated tryptophan and the alteration of AS by mutagenesis including single-nucleotide polymorphisms (SNPs) would enable the visualization and selection of over-accumulated mutant lines from the rice mutant population. Targeting Induced Local Lesions IN Genome analysis with five fluorescent-labelled primer sets covering the OASA1 locus resulted in the selection of three rice mutant lines, MRVII-9, MRVII-33 and MRVII-37. The tryptophan contents in the seed storage protein of the three mutant lines were elevated when compared with the wild-type cv. 'Dongan'. Genomic DNA of OASA1 was re-sequenced and validated at the SNP and other polymorphism sites. Three mutant lines (MRVII-9, MRVII-33 and MRVII-37) not only contained more than five SNP sites in the total regions of OASA1 gene, but also contained SNPs in coding regions that resulted in amino acid changes such as E241G, A560G and V495F/S496F, respectively. The SNPs, which resulted in amino acid changes, may affect the enhanced tryptophan content in the seed storage protein. These different features could utilize the selection of mutant lines with over-accumulated tryptophan from large rice mutant populations.

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