Abstract
In this work, we demonstrate in situ virus identification based on surface-enhanced Raman scattering (SERS). We hypothesized that newly emerging influenza viruses possess surface proteins and lipids that can generate distinctive Raman signals. To test this hypothesis, SERS signals were measured from the surface of a noninfluenza virus, two different influenza viruses, and a genetically shuffled influenza virus. To ensure the safety for experimenters we constructed nonreplicating pseudotyped viruses that display main influenza virus surface components. Pseudotype with influenza virus components produced enhanced Raman peaks, on gold nanoparticles, that are easily distinguishable from those of pseudotype with a noninfluenza virus component, vesicular stomatitis virus G protein (VSVG). Furthermore, virus with the surface components of a newly emerging influenza strain, A/California/04/2009 (H1N1), generated Raman peaks different from those of viruses with components of the conventional laboratory-adapted influenza strain, A/WSN/33 (H1N1). Interestingly, the virus simultaneously displaying surface components of both influenza strains, a model mutant with genome reassortment, also produced a Raman signal pattern that is clearly distinguishable from those of each strain. This work highlights that SERS can provide a powerful label-free strategy to quickly identify newly emerging and potentially fatal influenza viruses.
| Original language | English |
|---|---|
| Pages (from-to) | 11652-11659 |
| Number of pages | 8 |
| Journal | Analytical Chemistry |
| Volume | 87 |
| Issue number | 23 |
| DOIs | |
| Publication status | Published - 2015 Nov 3 |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Identification of Newly Emerging Influenza Viruses by Surface-Enhanced Raman Spectroscopy. / Lim, Jae Young; Nam, Jung Soo; Yang, Se Eun; Shin, Hyunku; Jang, Yoon Ha; Bae, Gyu Un; Kang, Taewook; Lim, Kwang Il; Choi, Yeonho.
In: Analytical Chemistry, Vol. 87, No. 23, 03.11.2015, p. 11652-11659.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Identification of Newly Emerging Influenza Viruses by Surface-Enhanced Raman Spectroscopy
AU - Lim, Jae Young
AU - Nam, Jung Soo
AU - Yang, Se Eun
AU - Shin, Hyunku
AU - Jang, Yoon Ha
AU - Bae, Gyu Un
AU - Kang, Taewook
AU - Lim, Kwang Il
AU - Choi, Yeonho
PY - 2015/11/3
Y1 - 2015/11/3
N2 - In this work, we demonstrate in situ virus identification based on surface-enhanced Raman scattering (SERS). We hypothesized that newly emerging influenza viruses possess surface proteins and lipids that can generate distinctive Raman signals. To test this hypothesis, SERS signals were measured from the surface of a noninfluenza virus, two different influenza viruses, and a genetically shuffled influenza virus. To ensure the safety for experimenters we constructed nonreplicating pseudotyped viruses that display main influenza virus surface components. Pseudotype with influenza virus components produced enhanced Raman peaks, on gold nanoparticles, that are easily distinguishable from those of pseudotype with a noninfluenza virus component, vesicular stomatitis virus G protein (VSVG). Furthermore, virus with the surface components of a newly emerging influenza strain, A/California/04/2009 (H1N1), generated Raman peaks different from those of viruses with components of the conventional laboratory-adapted influenza strain, A/WSN/33 (H1N1). Interestingly, the virus simultaneously displaying surface components of both influenza strains, a model mutant with genome reassortment, also produced a Raman signal pattern that is clearly distinguishable from those of each strain. This work highlights that SERS can provide a powerful label-free strategy to quickly identify newly emerging and potentially fatal influenza viruses.
AB - In this work, we demonstrate in situ virus identification based on surface-enhanced Raman scattering (SERS). We hypothesized that newly emerging influenza viruses possess surface proteins and lipids that can generate distinctive Raman signals. To test this hypothesis, SERS signals were measured from the surface of a noninfluenza virus, two different influenza viruses, and a genetically shuffled influenza virus. To ensure the safety for experimenters we constructed nonreplicating pseudotyped viruses that display main influenza virus surface components. Pseudotype with influenza virus components produced enhanced Raman peaks, on gold nanoparticles, that are easily distinguishable from those of pseudotype with a noninfluenza virus component, vesicular stomatitis virus G protein (VSVG). Furthermore, virus with the surface components of a newly emerging influenza strain, A/California/04/2009 (H1N1), generated Raman peaks different from those of viruses with components of the conventional laboratory-adapted influenza strain, A/WSN/33 (H1N1). Interestingly, the virus simultaneously displaying surface components of both influenza strains, a model mutant with genome reassortment, also produced a Raman signal pattern that is clearly distinguishable from those of each strain. This work highlights that SERS can provide a powerful label-free strategy to quickly identify newly emerging and potentially fatal influenza viruses.
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U2 - 10.1021/acs.analchem.5b02661
DO - 10.1021/acs.analchem.5b02661
M3 - Article
VL - 87
SP - 11652
EP - 11659
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 23
ER -