Identification of phage-displayed peptides which bind to the human HnRNPA1 protein-specific monoclonal antibody.

J. H. Kim, J. H. Lee, Joon Kim, Jeong Kook Kim

Research output: Contribution to journalArticle

Abstract

We have screened a peptide phage display library to examine if monoclonal antibody-binding phages could be isolated from the library and thereby predict the antigenic epitopes of the antibodies from the isolated phages. The library was screened for high-avidity binding to monoclonal antibodies by an affinity purification technique called biopanning. Among the monoclonal antibodies examined, the human hnRNPA1 protein-specific monoclonal antibody 9H10 showed selective binding of phages. After two rounds of the biopanning, twelve clones of high-avidity-binding phages were chosen and their inserts were sequenced. Nucleotide sequence comparison of the 12 clones showed that there were 5 different species, with two species containing four members, implying that they were predominantly selected by the biopanning. The amino acid sequences of the inserts of the 12 clones were compared with that of the human hnRNPA1 protein in order to find the putative epitope of the human hnRNPA1 protein for 9H10. The C-terminal region of the human hnRNPA1 protein shows significant homology with the peptide sequences of the selected phage clones. These results show that this peptide phage display library can be useful in defining the epitope of some monoclonal antibodies.

Original languageEnglish
Pages (from-to)452-454
Number of pages3
JournalMolecules and Cells
Volume9
Issue number4
Publication statusPublished - 1999 Aug 31

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Bacteriophages
Monoclonal Antibodies
Clone Cells
Peptides
Peptide Library
Epitopes
Proteins
Libraries
Antibody Affinity
Amino Acid Sequence
hnRNP A1
Antibodies

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Identification of phage-displayed peptides which bind to the human HnRNPA1 protein-specific monoclonal antibody. / Kim, J. H.; Lee, J. H.; Kim, Joon; Kim, Jeong Kook.

In: Molecules and Cells, Vol. 9, No. 4, 31.08.1999, p. 452-454.

Research output: Contribution to journalArticle

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