Identification of protein-arginine N-methyltransferase as 10- formyltetrahydrofolate dehydrogenase

Sangduk Kim, Gil Hong Park, Won A. Joo, Woon Ki Paik, Robert J. Cook, Kenneth R. Williams

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5 Citations (Scopus)


S-Adenosylmethionine:protein-arginine N-methyl-transferase (EC; protein methylase I) transfers the methyl group of S-adenosyl-L-methionine to an arginine residue of a protein substrate. The homogeneous liver protein methylase I was subjected to tryptic digestion followed by reverse phase high performance liquid chromatography (HPLC) separation and either 'on-line' mass spectrometric fragmentation or 'off-line' Edman sequencing of selected fractions. Data base searching of both the mass spectrometric and Edman sequencing data from several peptides identified the protein methylase as 10- formyltetrahydrofolate dehydrogenase (EC; Cook, R. J., Lloyd, R. S., and Wagner, C. (1991) J. Biol. Chem. 266, 4965-4973; Swiss accession number P28037). This identification was confirmed by comparative HPLC tryptic peptide mapping and affinity chromatography of the methylase on the 5- formyl-tetrahydrofolate-Sepharose affinity gel used to purify the dehydrogenase. The purified rat liver methylase had approximately 33% of the 10-formyltetrahydrofolate dehydrogenase and 36% of the aldehyde dehydrogenase activity as compared with the recombinant dehydrogenase, which also had protein methylase I activity. Polyclonal antibodies against recombinant dehydrogenase reacted with protein methylase I purified either by polyacrylamide gel electrophoresis or 5-formyltetrahydrofolate affinity chromatography. In each instance there was only a single immunoreactive band at a molecular weight of ~106,000. Together, these results confirm the co- identity of protein-arginine methyltransferase and 10-formyltetrahydrofolate dehydrogenase.

Original languageEnglish
Pages (from-to)27374-27382
Number of pages9
JournalJournal of Biological Chemistry
Issue number42
Publication statusPublished - 1998 Oct 16
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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