Identification of proteins expressed differently among surgically resected stage I lung adenocarcinomas

Eun Sil Ha, Seonyoung Choi, Kwang Ho In, Seung Hyeun Lee, Eun Joo Lee, Sang Yeub Lee, Je Hyeong Kim, Chol Shin, Jae Jeong Shim, Kyung Ho Kang, Sohee Phark, Donggeun Sul

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9 Citations (Scopus)

Abstract

Rationale: Among patients with surgically resected stage I lung adenocarcinoma, some succumb to early recurrence, while others survive for more than 5. years. Few markers to predict prognoses in these patients have been accepted. Recent advances in proteomic methodologies offer a unique chance to identify new candidate biomarkers. The aim of this study is to find differences in protein expression in resected lung cancer tissue of stage I adenocarcinoma from patients with no recurrence for more than 5. years and from those with early recurrence. Methods: Lung cancer tissues were obtained from 15 patients with pathologically confirmed stage I adenocarcinoma. The patients were divided into two groups, those with recurrence within 36. months (early recurrence group, n=. 9) and those that were disease-free for over 5. years (disease free group, n=. 6). Tissue proteins were separated by a two-dimensional electrophoresis long gel system (30. ×. 40. cm) with set ranges (3-10NL) and examined by nano-LC-ESI-MS/MS. Western blot assays were performed to validate these proteins. Results: Twelve protein spots were up-regulated and 8 were down-regulated in the disease-free group as compared with the recurrence group. Of the 12 up-regulated proteins, haptoglubin, tau-tubulin kinase-2 (TTBK2), thymidine phosphorylase, annexin-1, PIN1, CAPG, and SEC23 were validated by Western blot. Among the 8 down-regulated proteins, serpinB6 and trangelin-2 were validated. Conclusions: A total of 9 differentially expressed proteins were successfully extracted, identified, and confirmed from stage I lung adenocarcinoma tissues. The increased or decreased expression of these proteins according to prognosis may be the basis for further studies of proteomics in developing prognostic biomarkers.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalClinical Biochemistry
Volume46
Issue number4-5
DOIs
Publication statusPublished - 2013 Mar

Keywords

  • Biomarker
  • Lung adenocarcinoma
  • Prognosis
  • Proteomic analysis

ASJC Scopus subject areas

  • Clinical Biochemistry

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    Ha, E. S., Choi, S., In, K. H., Lee, S. H., Lee, E. J., Lee, S. Y., Kim, J. H., Shin, C., Shim, J. J., Kang, K. H., Phark, S., & Sul, D. (2013). Identification of proteins expressed differently among surgically resected stage I lung adenocarcinomas. Clinical Biochemistry, 46(4-5), 369-377. https://doi.org/10.1016/j.clinbiochem.2012.11.014