Identification of putative MAPK kinases in Oryza minuta and O. sativa responsive to biotic stresses

Min Kyoung You, Seung Ick Oh, Sung Han Ok, Sung Ki Cho, Hyun Young Shin, Ji Ung Jeung, Jeong Sheop Shin

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The mitogen-activated protein kinase (MAPK) signaling cascade is critical for regulating plant defense systems against various kinds of pathogen and environmental stresses. One component of this cascade, the MAP kinase kinases (MAPKK), has not yet been shown to be induced in plants following biotic attacks, such as those by insects and fungi. We describe here a gene coding for a blast (Magnaporthe grisea)- and insect (Nilaparvata lugens)-responsive putative MAPK kinase, OmMKK1 (Oryza minuta MAPKK1), which was identified in a library of O. minuta expressed sequence tags (ESTs). Two copies of OmMKK1 are present in the O. minuta genome. They encode a predicted protein with molecular mass 39 kDa and pI of 6.2. Transcript patterns following imbibition of plant hormones such as methyl jasmonic acid (MeJA), ethephone, salicylic acid (SA) and abscisic acid (ABA), as well as exposure to methyl viologen (MV), revealed that the expression of OmMKK1 is related to defense response signaling pathways. A comparative analysis of OmMKK1 and its O. sativa ortholog OsMKK1 showed that both were induced by stress-related hormones and biotic stresses, but that the kinetics of their responses differed despite their high amino acid sequence identity (96%).

Original languageEnglish
Pages (from-to)108-114
Number of pages7
JournalMolecules and Cells
Volume23
Issue number1
Publication statusPublished - 2007 Feb 1

Fingerprint

Mitogen-Activated Protein Kinase Kinases
Insects
Magnaporthe
Abscisic Acid
Paraquat
Plant Growth Regulators
Salicylic Acid
Expressed Sequence Tags
Mitogen-Activated Protein Kinases
Libraries
Amino Acid Sequence
Fungi
Genome
Hormones
Oryza
Genes
Proteins

Keywords

  • Blast
  • Brown planthopper
  • MAPK signaling cascade
  • Regulation of gene expression
  • Wild rice

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

You, M. K., Oh, S. I., Ok, S. H., Cho, S. K., Shin, H. Y., Jeung, J. U., & Shin, J. S. (2007). Identification of putative MAPK kinases in Oryza minuta and O. sativa responsive to biotic stresses. Molecules and Cells, 23(1), 108-114.

Identification of putative MAPK kinases in Oryza minuta and O. sativa responsive to biotic stresses. / You, Min Kyoung; Oh, Seung Ick; Ok, Sung Han; Cho, Sung Ki; Shin, Hyun Young; Jeung, Ji Ung; Shin, Jeong Sheop.

In: Molecules and Cells, Vol. 23, No. 1, 01.02.2007, p. 108-114.

Research output: Contribution to journalArticle

You, MK, Oh, SI, Ok, SH, Cho, SK, Shin, HY, Jeung, JU & Shin, JS 2007, 'Identification of putative MAPK kinases in Oryza minuta and O. sativa responsive to biotic stresses', Molecules and Cells, vol. 23, no. 1, pp. 108-114.
You MK, Oh SI, Ok SH, Cho SK, Shin HY, Jeung JU et al. Identification of putative MAPK kinases in Oryza minuta and O. sativa responsive to biotic stresses. Molecules and Cells. 2007 Feb 1;23(1):108-114.
You, Min Kyoung ; Oh, Seung Ick ; Ok, Sung Han ; Cho, Sung Ki ; Shin, Hyun Young ; Jeung, Ji Ung ; Shin, Jeong Sheop. / Identification of putative MAPK kinases in Oryza minuta and O. sativa responsive to biotic stresses. In: Molecules and Cells. 2007 ; Vol. 23, No. 1. pp. 108-114.
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