Identification of radiation-sensitive expressed genes in the ICR and AKR/J mouse thymus

Jin Jong Bong, Yu Mi Kang, Suk Chul Shin, Seung Jin Choi, Kyung-Mi Lee, Hee Sun Kim

Research output: Contribution to journalArticle

Abstract

We have investigated radiation-sensitive expressed genes (EGs), their signal pathways, and the effects of ionizing radiation in the thymus of ICR and AKR/J mice.Whole-body and relative thymus weights were taken and microarray analyses were done on the thymuses of high-dose-rate (HDR, 137Cs, 0.8 Gy/min, a single dose of 4.5 Gy) and low-dose-rate (LDR, 137Cs, 0.7 mGy/h, a cumulative dose of 1.7 Gy) irradiated ICR and AKR/J mice. Gene expression patterns were validated by quantitative polymerase chain reaction (qPCR). The effect of ionizing radiation on thymus cell apoptosis was measured terminal deoxynucleotidyltransferase-mediated dUTP-end labeling (TUNEL). LDR-irradiation increased the mean whole-body weight, but decreased the relative thymus weight of AKR/J mice. Radiation-sensitive EGs were found by comparing HDR- and LDR-irradiated ICR and AKR/J mice. qPCR analysis showed that 12 EGs had dose and dose-rate dependent expression patterns. Gene-network analysis indicated that Ighg, Igh-VJ558, Defb6, Reg3g, and Saa2 may be involved in the immune response, leukocyte migration, and apoptosis. Our data suggest that expression of the HDR (Glut1, Glut4, and PKLR) and LDR radiation-response genes (Ighg and Igh-VJ558) can be dose or dose-rate dependent. There was an increased number of apoptotic cells in HDR-irradiated ICR mice and LDR-irradiated AKR/J mice. Thus, changes of the mean whole-body weight and relative thymus weight, EGs, signal pathways, and the effects of ionizing radiation on the thymus of ICR and AKR/J mice are described.

Original languageEnglish
Pages (from-to)485-494
Number of pages10
JournalCell Biology International
Volume37
Issue number5
DOIs
Publication statusPublished - 2013 May 29

Fingerprint

Inbred AKR Mouse
Thymus Gland
Radiation
Genes
Ionizing Radiation
Weights and Measures
Signal Transduction
Body Weight
Apoptosis
Polymerase Chain Reaction
Inbred ICR Mouse
DNA Nucleotidylexotransferase
Gene Regulatory Networks
In Situ Nick-End Labeling
Microarray Analysis
Leukocytes
Cell Count
Gene Expression

Keywords

  • Apoptotic cells
  • Ionizing radiation
  • Microarray
  • Radiation-sensitive expressed genes
  • Thymus

ASJC Scopus subject areas

  • Cell Biology

Cite this

Identification of radiation-sensitive expressed genes in the ICR and AKR/J mouse thymus. / Bong, Jin Jong; Kang, Yu Mi; Shin, Suk Chul; Choi, Seung Jin; Lee, Kyung-Mi; Kim, Hee Sun.

In: Cell Biology International, Vol. 37, No. 5, 29.05.2013, p. 485-494.

Research output: Contribution to journalArticle

Bong, Jin Jong ; Kang, Yu Mi ; Shin, Suk Chul ; Choi, Seung Jin ; Lee, Kyung-Mi ; Kim, Hee Sun. / Identification of radiation-sensitive expressed genes in the ICR and AKR/J mouse thymus. In: Cell Biology International. 2013 ; Vol. 37, No. 5. pp. 485-494.
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