Identification of the αvβ3 integrin-interacting motif of βig-h3 and its anti-angiogenic effect

Ju Ock Nam, Jung Eun Kim, Ha Won Jeong, Sung Jin Lee, Byung Heon Lee, Je Yong Choi, Rang Woon Park, Jae Yong Park, In San Kim

Research output: Contribution to journalArticle

106 Citations (Scopus)

Abstract

βig-h3 is an extracellular matrix protein that mediates adhesion and migration of several cell types through interaction with integrins. In the present study, we tested whether βig-h3 mediates endothelial cell adhesion and migration, thereby regulating angiogenesis. In this study, we demonstrate that not only βig-h3 itself but also all four fas-1 domains of βig-h3 mediate endothelial cell adhesion and migration through interaction with the αvβ3 integrin. We found that the αvβ3 integrin-interacting motif of the four fas-1 domains of βig-h3 is the same YH motif that we reported previously to interact with αvβ5 integrin. The YH peptide inhibited endothelial cell adhesion and migration in a dose-dependent manner. We demonstrate that the YH peptide has anti-angiogenic activity in vitro and in vivo using an endothelial cell tube formation assay and a Matrigel plug assay, respectively. Our results reveal that βig-h3 bears αvβ3 integrin-interacting motifs that mediate endothelial cell adhesion and migration and, therefore, may regulate angiogenesis.

Original languageEnglish
Pages (from-to)25902-25909
Number of pages8
JournalJournal of Biological Chemistry
Volume278
Issue number28
DOIs
Publication statusPublished - 2003 Jul 11
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Identification of the α<sub>v</sub>β<sub>3</sub> integrin-interacting motif of βig-h3 and its anti-angiogenic effect'. Together they form a unique fingerprint.

  • Cite this

    Nam, J. O., Kim, J. E., Jeong, H. W., Lee, S. J., Lee, B. H., Choi, J. Y., Park, R. W., Park, J. Y., & Kim, I. S. (2003). Identification of the αvβ3 integrin-interacting motif of βig-h3 and its anti-angiogenic effect. Journal of Biological Chemistry, 278(28), 25902-25909. https://doi.org/10.1074/jbc.M300358200