Identification of the αvβ3 integrin-interacting motif of βig-h3 and its anti-angiogenic effect

Ju Ock Nam, Jung Eun Kim, Ha Won Jeong, Sung Jin Lee, Byung Heon Lee, Je Yong Choi, Rang Woon Park, Jae Yong Park, In-San Kim

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105 Citations (Scopus)

Abstract

βig-h3 is an extracellular matrix protein that mediates adhesion and migration of several cell types through interaction with integrins. In the present study, we tested whether βig-h3 mediates endothelial cell adhesion and migration, thereby regulating angiogenesis. In this study, we demonstrate that not only βig-h3 itself but also all four fas-1 domains of βig-h3 mediate endothelial cell adhesion and migration through interaction with the αvβ3 integrin. We found that the αvβ3 integrin-interacting motif of the four fas-1 domains of βig-h3 is the same YH motif that we reported previously to interact with αvβ5 integrin. The YH peptide inhibited endothelial cell adhesion and migration in a dose-dependent manner. We demonstrate that the YH peptide has anti-angiogenic activity in vitro and in vivo using an endothelial cell tube formation assay and a Matrigel plug assay, respectively. Our results reveal that βig-h3 bears αvβ3 integrin-interacting motifs that mediate endothelial cell adhesion and migration and, therefore, may regulate angiogenesis.

Original languageEnglish
Pages (from-to)25902-25909
Number of pages8
JournalJournal of Biological Chemistry
Volume278
Issue number28
DOIs
Publication statusPublished - 2003 Jul 11
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Nam, J. O., Kim, J. E., Jeong, H. W., Lee, S. J., Lee, B. H., Choi, J. Y., Park, R. W., Park, J. Y., & Kim, I-S. (2003). Identification of the αvβ3 integrin-interacting motif of βig-h3 and its anti-angiogenic effect. Journal of Biological Chemistry, 278(28), 25902-25909. https://doi.org/10.1074/jbc.M300358200