Identification of the cis-element and bZIP DNA binding motifs for the autogenous negative control of mouse NOSTRIN

Seong Ho Bae, Young Joon Choi, Kyung Hyun Kim, Sung Soo Park

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

mNOSTRIN is the mouse ortholog of hNOSTRIN. Unlike hNOSTRIN, which is alternatively spliced to produce two isoforms (α and β), only a single isoform of mNOSTRIN has been detected in either the nucleus or cytoplasm/membrane. Because mNOSTRIN represses its own transcription through direct binding onto its own promoter, this protein is constantly expressed in a temporally regulated pattern during differentiation of F9 embryonic carcinoma cells. In this study, we identified the specific cis-element in the mNOSTRIN regulatory region that is responsible for negative autogenous control. This element exhibits inverted dyad symmetry. Furthermore, we identified a putative bZIP motif in the middle region of mNOSTRIN, which is responsible for DNA binding, and showed that disruption of the leucine zippers abolished the DNA-binding activity of mNOSTRIN. Here, we report that a single form of mNOSTRIN functions in both the nucleus and cytoplasm/membrane. In the nucleus, mNOSTRIN acts as a transcriptional repressor by binding to the cis-element through its bZIP motif.

Original languageEnglish
Pages (from-to)924-931
Number of pages8
JournalBiochemical and biophysical research communications
Volume443
Issue number3
DOIs
Publication statusPublished - 2014 Jan 17

Keywords

  • Inverted-repeat
  • NOSTRIN
  • bZIP

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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