Identification of the lactic acid bacteria in kimchi according to initial and over-ripened fermentation using PCR and 16S rRNA gene sequence analysis

Jung Min Park, Jin Ho Shin, Dan Won Lee, Jae Chul Song, Hyung Joo Suh, Un Jae Chang, Jin Man Kim

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

This paper aimed to identify the lactic acid bacteria species involved in kimchi fermentation at different fermentation periods by using culture-independent 16S rRNA gene clone libraries, and develop polymerase chain reaction for the detection of lactic acid bacteria (LAB). We investigated 6 commercially produced kimchi samples, including kimchi at an initial stage of fermentation and kimchi that was fermented to an over-ripened stage. The results of our study show that the analysis with cultureindependent 16S rRNA gene clone libraries could successfully identify 134 clones, 11 species, including Weissella, Lactobacillus, Pediococcus, and Leuconostoc from the 6 commercial kimchi samples. Weisella koreensis and Lactobacillus brevis were the predominant LAB in the initial stage of kimchi fermentation at 4°C (pH 4.96-5.27 and acidity 0.81-0.88), and Leuconostoc gelidum and Lactobacillus sakei subsp. sakei may play an important role in kimchi fermentation at the over-ripened stage (pH 3.61-3.91 and acidity 1.70-1.79).

Original languageEnglish
Pages (from-to)541-546
Number of pages6
JournalFood Science and Biotechnology
Volume19
Issue number2
DOIs
Publication statusPublished - 2010 Apr 1

Fingerprint

kimchi
rRNA Genes
Fermentation
lactic acid bacteria
Sequence Analysis
Lactic Acid
sequence analysis
fermentation
ribosomal RNA
Bacteria
nucleotide sequences
Polymerase Chain Reaction
Leuconostoc
Clone Cells
Gene Library
Leuconostocaceae
Weissella
Lactobacillus brevis
clones
Pediococcus

Keywords

  • 16S rRNA sequence analysis
  • Kimchi
  • Lactic acid bacteria
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Food Science
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Identification of the lactic acid bacteria in kimchi according to initial and over-ripened fermentation using PCR and 16S rRNA gene sequence analysis. / Park, Jung Min; Shin, Jin Ho; Lee, Dan Won; Song, Jae Chul; Suh, Hyung Joo; Chang, Un Jae; Kim, Jin Man.

In: Food Science and Biotechnology, Vol. 19, No. 2, 01.04.2010, p. 541-546.

Research output: Contribution to journalArticle

Park, Jung Min ; Shin, Jin Ho ; Lee, Dan Won ; Song, Jae Chul ; Suh, Hyung Joo ; Chang, Un Jae ; Kim, Jin Man. / Identification of the lactic acid bacteria in kimchi according to initial and over-ripened fermentation using PCR and 16S rRNA gene sequence analysis. In: Food Science and Biotechnology. 2010 ; Vol. 19, No. 2. pp. 541-546.
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