Identification of Tumor Initiating Cells with a Small-Molecule Fluorescent Probe by Using Vimentin as a Biomarker

Yong An Lee; Jong Jin Kim; Jungyeol Lee; Jia Hui Jane Lee; Srikanta Sahu; Haw Young Kwon; Sung Jin Park; Se Young Jang; Jun Seok Lee; Zhenxun Wang; Wai Leong Tam; Bing Lim; Nam Young Kang; Young Tae Chang

doi:10.1002/anie.201712920

Identification of Tumor Initiating Cells with a Small-Molecule Fluorescent Probe by Using Vimentin as a Biomarker

Yong An Lee, Jong Jin Kim, Jungyeol Lee, Jia Hui Jane Lee, Srikanta Sahu, Haw Young Kwon, Sung Jin Park, Se Young Jang, Jun Seok Lee, Zhenxun Wang, Wai Leong Tam, Bing Lim, Nam Young Kang, Young Tae Chang

Research output: Contribution to journal › Article › peer-review

27 Citations (Scopus)

Abstract

Tumor initiating cells (TICs) have been implicated in clinical relapse and metastasis of a variety of epithelial cancers, including lung cancer. While efforts toward the development of specific probes for TIC detection and targeting are ongoing, a universal TIC probe has yet to be developed. We report the first TIC-specific fluorescent chemical probe, TiY, with identification of the molecular target as vimentin, a marker for epithelial-to-mesenchymal transition (EMT). TiY selectively stains TICs over differentiated tumor cells or normal cells, and facilitates the visualization and enrichment of functionally active TICs from patient tumors. At high concentration, TiY also shows anti-TIC activity with low toxicity to non-TICs. With the unexplored target vimentin, TiY shows potential as a first universal probe for TIC detection in different cancers.

Original language	English
Pages (from-to)	2851-2854
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	57
Issue number	11
DOIs	https://doi.org/10.1002/anie.201712920
Publication status	Published - 2018 Mar 5
Externally published	Yes

Keywords

antitumor agents
biomarkers
cancer
fluorescent probes
high-throughput screening

ASJC Scopus subject areas

Catalysis
Chemistry(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/anie.201712920

Cite this

Lee, Y. A., Kim, J. J., Lee, J., Lee, J. H. J., Sahu, S., Kwon, H. Y., Park, S. J., Jang, S. Y., Lee, J. S., Wang, Z., Tam, W. L., Lim, B., Kang, N. Y., & Chang, Y. T. (2018). Identification of Tumor Initiating Cells with a Small-Molecule Fluorescent Probe by Using Vimentin as a Biomarker. Angewandte Chemie - International Edition, 57(11), 2851-2854. https://doi.org/10.1002/anie.201712920

@article{8024234cd6f844749569cb93b5980328,

title = "Identification of Tumor Initiating Cells with a Small-Molecule Fluorescent Probe by Using Vimentin as a Biomarker",

abstract = "Tumor initiating cells (TICs) have been implicated in clinical relapse and metastasis of a variety of epithelial cancers, including lung cancer. While efforts toward the development of specific probes for TIC detection and targeting are ongoing, a universal TIC probe has yet to be developed. We report the first TIC-specific fluorescent chemical probe, TiY, with identification of the molecular target as vimentin, a marker for epithelial-to-mesenchymal transition (EMT). TiY selectively stains TICs over differentiated tumor cells or normal cells, and facilitates the visualization and enrichment of functionally active TICs from patient tumors. At high concentration, TiY also shows anti-TIC activity with low toxicity to non-TICs. With the unexplored target vimentin, TiY shows potential as a first universal probe for TIC detection in different cancers.",

keywords = "antitumor agents, biomarkers, cancer, fluorescent probes, high-throughput screening",

author = "Lee, {Yong An} and Kim, {Jong Jin} and Jungyeol Lee and Lee, {Jia Hui Jane} and Srikanta Sahu and Kwon, {Haw Young} and Park, {Sung Jin} and Jang, {Se Young} and Lee, {Jun Seok} and Zhenxun Wang and Tam, {Wai Leong} and Bing Lim and Kang, {Nam Young} and Chang, {Young Tae}",

note = "Funding Information: This study was supported by an intramural funding from the A*STAR (Agency for Science, Technology and Research, Singapore) Biomedical Research Council, Institute for Basic Science (IBS) IBS-R007-A1, and the National Medical Research Council grant NMRC/TCR/016-NNI/2016. We thank SBIC-Nikon Imaging Centre for confocal microscopy facilities. Publisher Copyright: {\textcopyright} 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2018",

month = mar,

day = "5",

doi = "10.1002/anie.201712920",

language = "English",

volume = "57",

pages = "2851--2854",

journal = "Angewandte Chemie - International Edition",

issn = "1433-7851",

publisher = "John Wiley and Sons Ltd",

number = "11",

}

TY - JOUR

T1 - Identification of Tumor Initiating Cells with a Small-Molecule Fluorescent Probe by Using Vimentin as a Biomarker

AU - Lee, Yong An

AU - Kim, Jong Jin

AU - Lee, Jungyeol

AU - Lee, Jia Hui Jane

AU - Sahu, Srikanta

AU - Kwon, Haw Young

AU - Park, Sung Jin

AU - Jang, Se Young

AU - Lee, Jun Seok

AU - Wang, Zhenxun

AU - Tam, Wai Leong

AU - Lim, Bing

AU - Kang, Nam Young

AU - Chang, Young Tae

N1 - Funding Information: This study was supported by an intramural funding from the A*STAR (Agency for Science, Technology and Research, Singapore) Biomedical Research Council, Institute for Basic Science (IBS) IBS-R007-A1, and the National Medical Research Council grant NMRC/TCR/016-NNI/2016. We thank SBIC-Nikon Imaging Centre for confocal microscopy facilities. Publisher Copyright: © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

PY - 2018/3/5

Y1 - 2018/3/5

N2 - Tumor initiating cells (TICs) have been implicated in clinical relapse and metastasis of a variety of epithelial cancers, including lung cancer. While efforts toward the development of specific probes for TIC detection and targeting are ongoing, a universal TIC probe has yet to be developed. We report the first TIC-specific fluorescent chemical probe, TiY, with identification of the molecular target as vimentin, a marker for epithelial-to-mesenchymal transition (EMT). TiY selectively stains TICs over differentiated tumor cells or normal cells, and facilitates the visualization and enrichment of functionally active TICs from patient tumors. At high concentration, TiY also shows anti-TIC activity with low toxicity to non-TICs. With the unexplored target vimentin, TiY shows potential as a first universal probe for TIC detection in different cancers.

AB - Tumor initiating cells (TICs) have been implicated in clinical relapse and metastasis of a variety of epithelial cancers, including lung cancer. While efforts toward the development of specific probes for TIC detection and targeting are ongoing, a universal TIC probe has yet to be developed. We report the first TIC-specific fluorescent chemical probe, TiY, with identification of the molecular target as vimentin, a marker for epithelial-to-mesenchymal transition (EMT). TiY selectively stains TICs over differentiated tumor cells or normal cells, and facilitates the visualization and enrichment of functionally active TICs from patient tumors. At high concentration, TiY also shows anti-TIC activity with low toxicity to non-TICs. With the unexplored target vimentin, TiY shows potential as a first universal probe for TIC detection in different cancers.

KW - antitumor agents

KW - biomarkers

KW - cancer

KW - fluorescent probes

KW - high-throughput screening

UR - http://www.scopus.com/inward/record.url?scp=85042037310&partnerID=8YFLogxK

U2 - 10.1002/anie.201712920

DO - 10.1002/anie.201712920

M3 - Article

C2 - 29377425

AN - SCOPUS:85042037310

VL - 57

SP - 2851

EP - 2854

JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

SN - 1433-7851

IS - 11

ER -

Identification of Tumor Initiating Cells with a Small-Molecule Fluorescent Probe by Using Vimentin as a Biomarker

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this