Identification of up-regulated proteins in the hemolymph of immunized Bombyx mori larvae

Kyung Han Song, Su Jin Jung, Young R. Seo, Seok Woo Kang, Sung Sik Han

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Insects defend themselves against foreign invaders via both a cellular response and a humoral response. The objective of this study was to identify proteins which were differently regulated in the immunized Bombyx mori larvae. Heat-inactivated bacteria (Bacillus megaterium) were injected into B. mori larvae, 4 days after final ecdysis. After 6 h, we identified the immune proteins in the hemolymph which had been differentially regulated in the immune-challenged larvae, using two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and quadrupole time-of-flight (Q-TOF) tandem mass spectrometry (MS). After the bacterial injection, more than 30 spots determined to have been up-regulated, and 11 spots were down-regulated. The heat shock 70 kDa protein cognate was one of the up-regulated hemocytic proteins, and peptidoglycan recognition protein, antichymotrypsin precursor, and gloverin-like protein 1∼4 were newly synthesized in the plasma. Antennal binding protein 7 was up-regulated in the plasma. Our results indicated that these immune response proteins were involved with the carrying out of innate immune responses.

Original languageEnglish
Pages (from-to)260-266
Number of pages7
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume1
Issue number2
DOIs
Publication statusPublished - 2006 Jun 1

Fingerprint

Bombyx
Hemolymph
Larva
Proteins
HSC70 Heat-Shock Proteins
Plasmas
Bacillus megaterium
Bacilli
Molting
Electrophoresis
Protein Precursors
Mass spectrometry
Electrophoresis, Gel, Two-Dimensional
Bacteria
Carrier Proteins
Tandem Mass Spectrometry
Innate Immunity
Insects
Hot Temperature
Injections

Keywords

  • Antennal binding protein 7
  • Antichymotrypsin
  • Bombyx mori
  • Gloverin
  • Heat shock 70 kDa protein (Hsp70)
  • Immune response proteins
  • Innate immunity
  • Peptidoglycan recognition protein (PGRP)
  • Proteomic analysis

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology
  • Physiology

Cite this

Identification of up-regulated proteins in the hemolymph of immunized Bombyx mori larvae. / Song, Kyung Han; Jung, Su Jin; Seo, Young R.; Kang, Seok Woo; Han, Sung Sik.

In: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Vol. 1, No. 2, 01.06.2006, p. 260-266.

Research output: Contribution to journalArticle

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