Identification of Yersinia enterocolitica using a random genomic DNA microarray chip

J. Bang; L. R. Beuchat; M. B. Gu; H. I. Chang; J. H. Ryu

doi:10.1111/j.1472-765X.2010.02954.x

Identification of Yersinia enterocolitica using a random genomic DNA microarray chip

J. Bang, L. R. Beuchat, M. B. Gu, H. I. Chang, J. H. Ryu

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Aims: To fabricate a DNA chip containing random fragments of genomic DNA of Yersinia enterocolitica and to verify its diagnostic ability.Methods and Results: A DNA microarray chip was fabricated using randomly fragmented DNA of Y. enterocolitica. Chips were hybridized with genomic DNA extracted from other Y. enterocolitica strains, other Yersinia spp. and bacteria in different genera. Genomic DNA extracted from Y. enterocolitica showed a significantly higher hybridization rate compared with DNA of other Yersinia spp. or bacterial genera, thereby distinguishing it from other bacteria.Conclusions: A DNA chip containing randomly fragmented genomic DNA from Y.enterocolitica can detect Y.enterocolitica and clearly distinguish it from other Yersinia spp. and bacteria in different genera.Significance and Impact of the Study: A microarray chip containing randomly fragmented genomic DNA of Y.enterocolitica was fabricated without sequence information, and its diagnostic ability to identify Y.enterocolitica was verified.

Original language	English
Pages (from-to)	665-670
Number of pages	6
Journal	Letters in Applied Microbiology
Volume	51
Issue number	6
DOIs	https://doi.org/10.1111/j.1472-765X.2010.02954.x
Publication status	Published - 2010 Dec

Keywords

DNA chip
Identification
Randomly fragmented genomic DNA
Yersinia enterocolitica

ASJC Scopus subject areas

Applied Microbiology and Biotechnology

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10.1111/j.1472-765X.2010.02954.x

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title = "Identification of Yersinia enterocolitica using a random genomic DNA microarray chip",

abstract = "Aims: To fabricate a DNA chip containing random fragments of genomic DNA of Yersinia enterocolitica and to verify its diagnostic ability.Methods and Results: A DNA microarray chip was fabricated using randomly fragmented DNA of Y. enterocolitica. Chips were hybridized with genomic DNA extracted from other Y. enterocolitica strains, other Yersinia spp. and bacteria in different genera. Genomic DNA extracted from Y. enterocolitica showed a significantly higher hybridization rate compared with DNA of other Yersinia spp. or bacterial genera, thereby distinguishing it from other bacteria.Conclusions: A DNA chip containing randomly fragmented genomic DNA from Y.enterocolitica can detect Y.enterocolitica and clearly distinguish it from other Yersinia spp. and bacteria in different genera.Significance and Impact of the Study: A microarray chip containing randomly fragmented genomic DNA of Y.enterocolitica was fabricated without sequence information, and its diagnostic ability to identify Y.enterocolitica was verified.",

keywords = "DNA chip, Identification, Randomly fragmented genomic DNA, Yersinia enterocolitica",

author = "J. Bang and Beuchat, {L. R.} and Gu, {M. B.} and Chang, {H. I.} and Ryu, {J. H.}",

year = "2010",

month = dec,

doi = "10.1111/j.1472-765X.2010.02954.x",

language = "English",

volume = "51",

pages = "665--670",

journal = "Letters in Applied Microbiology",

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publisher = "Wiley-Blackwell",

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TY - JOUR

T1 - Identification of Yersinia enterocolitica using a random genomic DNA microarray chip

AU - Bang, J.

AU - Beuchat, L. R.

AU - Gu, M. B.

AU - Chang, H. I.

AU - Ryu, J. H.

PY - 2010/12

Y1 - 2010/12

N2 - Aims: To fabricate a DNA chip containing random fragments of genomic DNA of Yersinia enterocolitica and to verify its diagnostic ability.Methods and Results: A DNA microarray chip was fabricated using randomly fragmented DNA of Y. enterocolitica. Chips were hybridized with genomic DNA extracted from other Y. enterocolitica strains, other Yersinia spp. and bacteria in different genera. Genomic DNA extracted from Y. enterocolitica showed a significantly higher hybridization rate compared with DNA of other Yersinia spp. or bacterial genera, thereby distinguishing it from other bacteria.Conclusions: A DNA chip containing randomly fragmented genomic DNA from Y.enterocolitica can detect Y.enterocolitica and clearly distinguish it from other Yersinia spp. and bacteria in different genera.Significance and Impact of the Study: A microarray chip containing randomly fragmented genomic DNA of Y.enterocolitica was fabricated without sequence information, and its diagnostic ability to identify Y.enterocolitica was verified.

AB - Aims: To fabricate a DNA chip containing random fragments of genomic DNA of Yersinia enterocolitica and to verify its diagnostic ability.Methods and Results: A DNA microarray chip was fabricated using randomly fragmented DNA of Y. enterocolitica. Chips were hybridized with genomic DNA extracted from other Y. enterocolitica strains, other Yersinia spp. and bacteria in different genera. Genomic DNA extracted from Y. enterocolitica showed a significantly higher hybridization rate compared with DNA of other Yersinia spp. or bacterial genera, thereby distinguishing it from other bacteria.Conclusions: A DNA chip containing randomly fragmented genomic DNA from Y.enterocolitica can detect Y.enterocolitica and clearly distinguish it from other Yersinia spp. and bacteria in different genera.Significance and Impact of the Study: A microarray chip containing randomly fragmented genomic DNA of Y.enterocolitica was fabricated without sequence information, and its diagnostic ability to identify Y.enterocolitica was verified.

KW - DNA chip

KW - Identification

KW - Randomly fragmented genomic DNA

KW - Yersinia enterocolitica

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DO - 10.1111/j.1472-765X.2010.02954.x

M3 - Article

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AN - SCOPUS:78349286745

SN - 0266-8254

VL - 51

SP - 665

EP - 670

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

IS - 6

ER -

Identification of Yersinia enterocolitica using a random genomic DNA microarray chip

Abstract

Keywords

ASJC Scopus subject areas

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