Identification of Yersinia enterocolitica using a random genomic DNA microarray chip

J. Bang, L. R. Beuchat, Man Bock Gu, Hyo-Ihl Chang, Jee-Hoon Ryu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Aims: To fabricate a DNA chip containing random fragments of genomic DNA of Yersinia enterocolitica and to verify its diagnostic ability.Methods and Results: A DNA microarray chip was fabricated using randomly fragmented DNA of Y. enterocolitica. Chips were hybridized with genomic DNA extracted from other Y. enterocolitica strains, other Yersinia spp. and bacteria in different genera. Genomic DNA extracted from Y. enterocolitica showed a significantly higher hybridization rate compared with DNA of other Yersinia spp. or bacterial genera, thereby distinguishing it from other bacteria.Conclusions: A DNA chip containing randomly fragmented genomic DNA from Y.enterocolitica can detect Y.enterocolitica and clearly distinguish it from other Yersinia spp. and bacteria in different genera.Significance and Impact of the Study: A microarray chip containing randomly fragmented genomic DNA of Y.enterocolitica was fabricated without sequence information, and its diagnostic ability to identify Y.enterocolitica was verified.

Original languageEnglish
Pages (from-to)665-670
Number of pages6
JournalLetters in Applied Microbiology
Volume51
Issue number6
DOIs
Publication statusPublished - 2010 Dec 1

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Yersinia enterocolitica
Oligonucleotide Array Sequence Analysis
DNA
Yersinia
Bacteria

Keywords

  • DNA chip
  • Identification
  • Randomly fragmented genomic DNA
  • Yersinia enterocolitica

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Identification of Yersinia enterocolitica using a random genomic DNA microarray chip. / Bang, J.; Beuchat, L. R.; Gu, Man Bock; Chang, Hyo-Ihl; Ryu, Jee-Hoon.

In: Letters in Applied Microbiology, Vol. 51, No. 6, 01.12.2010, p. 665-670.

Research output: Contribution to journalArticle

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AU - Beuchat, L. R.

AU - Gu, Man Bock

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AU - Ryu, Jee-Hoon

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N2 - Aims: To fabricate a DNA chip containing random fragments of genomic DNA of Yersinia enterocolitica and to verify its diagnostic ability.Methods and Results: A DNA microarray chip was fabricated using randomly fragmented DNA of Y. enterocolitica. Chips were hybridized with genomic DNA extracted from other Y. enterocolitica strains, other Yersinia spp. and bacteria in different genera. Genomic DNA extracted from Y. enterocolitica showed a significantly higher hybridization rate compared with DNA of other Yersinia spp. or bacterial genera, thereby distinguishing it from other bacteria.Conclusions: A DNA chip containing randomly fragmented genomic DNA from Y.enterocolitica can detect Y.enterocolitica and clearly distinguish it from other Yersinia spp. and bacteria in different genera.Significance and Impact of the Study: A microarray chip containing randomly fragmented genomic DNA of Y.enterocolitica was fabricated without sequence information, and its diagnostic ability to identify Y.enterocolitica was verified.

AB - Aims: To fabricate a DNA chip containing random fragments of genomic DNA of Yersinia enterocolitica and to verify its diagnostic ability.Methods and Results: A DNA microarray chip was fabricated using randomly fragmented DNA of Y. enterocolitica. Chips were hybridized with genomic DNA extracted from other Y. enterocolitica strains, other Yersinia spp. and bacteria in different genera. Genomic DNA extracted from Y. enterocolitica showed a significantly higher hybridization rate compared with DNA of other Yersinia spp. or bacterial genera, thereby distinguishing it from other bacteria.Conclusions: A DNA chip containing randomly fragmented genomic DNA from Y.enterocolitica can detect Y.enterocolitica and clearly distinguish it from other Yersinia spp. and bacteria in different genera.Significance and Impact of the Study: A microarray chip containing randomly fragmented genomic DNA of Y.enterocolitica was fabricated without sequence information, and its diagnostic ability to identify Y.enterocolitica was verified.

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