IgA+ plasma cells in murine intestinal lamina propria as a positive regulator of Treg differentiation

Myun Soo Kim, Tae Sung Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Continuous exposure to commensal bacteria gives rise to a complex intestinal immune system that maintains local tolerance, which requires Foxp3-expressing Treg. Recently, the regulation of TFH function by plasma cells has been reported, but effects of intestinal LP-PCs, one of the richest plasma cells in the body, on T cell differentiation have not been studied. Here, we investigated whether IgA+ LP-PCs from murine small intestines had effects on T cell differentiation. Surprisingly, when IgA+ LP-PCs were cocultured with CD4+ T cells, Foxp3 expression was increased significantly in CD4+CD25- T cells. Results using the Transwell coculture system revealed that soluble factors from LP-PCs, TGF-β, and RA were involved in the induction of Foxp3 expression. Furthermore, Foxp3+CD25- T cells were decreased in PP after intestinal depletion of plasma cells. In addition, intestinal colony transfer from SPF to germ-free mice was demonstrated to generate IgA+ LP-PCs and Foxp3+ T cells with meaningful correlation in LP. We report for the first time that IgA+ LP-PCs induce Foxp3 expression in T cells through TGF-β and RA. LP-PCs generated by commensal bacteria may play a crucial role in intestinal immunity through the induction of Treg, as well as IgA production.

Original languageEnglish
Pages (from-to)461-469
Number of pages9
JournalJournal of Leukocyte Biology
Volume95
Issue number3
DOIs
Publication statusPublished - 2014 Jan 1

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Plasma Cells
Immunoglobulin A
Mucous Membrane
T-Lymphocytes
Cell Differentiation
Bacteria
Coculture Techniques
Small Intestine
Immune System
Immunity

Keywords

  • Foxp3
  • Regulatory T cell
  • Retinoic acid
  • TGF-β

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

IgA+ plasma cells in murine intestinal lamina propria as a positive regulator of Treg differentiation. / Kim, Myun Soo; Kim, Tae Sung.

In: Journal of Leukocyte Biology, Vol. 95, No. 3, 01.01.2014, p. 461-469.

Research output: Contribution to journalArticle

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