IL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activity

Jeongsim Hue, Ankeun Kim, Hyunkeun Song, Inpo Choi, Hyunjeong Park, Taesung Kim, Wang Jae Lee, Hyungsik Kang, Daeho Cho

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

It has been reported that interleukin-18 (IL-18) is secreted by B16 murine melanoma cells and that this endogenous IL-18 is involved in the immune escape of murine melanoma cells. The present study investigated whether interleukin (IL)-18 can regulate stem cell factor (SCF) expression, known to be associated with melanocyte proliferation, in B16F10 murine melanoma cells. SCF expression was examined by RT-PCR, intracellular FACS analysis, and ELISA in IL-18 antisense transfectants. Transfection with IL-18 antisense cDNA reduced SCF expression and the expression was enhanced by addition of exogenous IL-18. In addition, the effect of IL-18 was blocked by the antioxidant, N-acetyl-l-cysteine (NAC), indicating that IL-18 regulates ROI production, which is involved in SCF production. Furthermore, inhibitors of p38 mitogen-activated protein kinase (MAPK), such as SB203580, blocked enhanced SCF expression, indicating that p38 MAPK activity is required for IL-18-enhanced SCF production. Taken together, these results suggest that IL-18 plays a critical role as a regulatory factor of SCF expression via ROI and p38 MAPK activity in B16F10 murine melanoma cells.

Original languageEnglish
Pages (from-to)211-217
Number of pages7
JournalImmunology Letters
Volume96
Issue number2
DOIs
Publication statusPublished - 2005 Jan 31
Externally publishedYes

Keywords

  • IL-18
  • Melanoma
  • ROI
  • SCF
  • p38 MAPK

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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