TY - JOUR
T1 - IL-18 enhances SCF production of melanoma cells by regulating ROI and p38 MAPK activity
AU - Hue, Jeongsim
AU - Kim, Ankeun
AU - Song, Hyunkeun
AU - Choi, Inpo
AU - Park, Hyunjeong
AU - Kim, Taesung
AU - Lee, Wang Jae
AU - Kang, Hyungsik
AU - Cho, Daeho
N1 - Funding Information:
This work was supported by Korea Research Foundation Grant (KRF-2002-005-C00015).
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2005/1/31
Y1 - 2005/1/31
N2 - It has been reported that interleukin-18 (IL-18) is secreted by B16 murine melanoma cells and that this endogenous IL-18 is involved in the immune escape of murine melanoma cells. The present study investigated whether interleukin (IL)-18 can regulate stem cell factor (SCF) expression, known to be associated with melanocyte proliferation, in B16F10 murine melanoma cells. SCF expression was examined by RT-PCR, intracellular FACS analysis, and ELISA in IL-18 antisense transfectants. Transfection with IL-18 antisense cDNA reduced SCF expression and the expression was enhanced by addition of exogenous IL-18. In addition, the effect of IL-18 was blocked by the antioxidant, N-acetyl-l-cysteine (NAC), indicating that IL-18 regulates ROI production, which is involved in SCF production. Furthermore, inhibitors of p38 mitogen-activated protein kinase (MAPK), such as SB203580, blocked enhanced SCF expression, indicating that p38 MAPK activity is required for IL-18-enhanced SCF production. Taken together, these results suggest that IL-18 plays a critical role as a regulatory factor of SCF expression via ROI and p38 MAPK activity in B16F10 murine melanoma cells.
AB - It has been reported that interleukin-18 (IL-18) is secreted by B16 murine melanoma cells and that this endogenous IL-18 is involved in the immune escape of murine melanoma cells. The present study investigated whether interleukin (IL)-18 can regulate stem cell factor (SCF) expression, known to be associated with melanocyte proliferation, in B16F10 murine melanoma cells. SCF expression was examined by RT-PCR, intracellular FACS analysis, and ELISA in IL-18 antisense transfectants. Transfection with IL-18 antisense cDNA reduced SCF expression and the expression was enhanced by addition of exogenous IL-18. In addition, the effect of IL-18 was blocked by the antioxidant, N-acetyl-l-cysteine (NAC), indicating that IL-18 regulates ROI production, which is involved in SCF production. Furthermore, inhibitors of p38 mitogen-activated protein kinase (MAPK), such as SB203580, blocked enhanced SCF expression, indicating that p38 MAPK activity is required for IL-18-enhanced SCF production. Taken together, these results suggest that IL-18 plays a critical role as a regulatory factor of SCF expression via ROI and p38 MAPK activity in B16F10 murine melanoma cells.
KW - IL-18
KW - Melanoma
KW - ROI
KW - SCF
KW - p38 MAPK
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U2 - 10.1016/j.imlet.2004.08.008
DO - 10.1016/j.imlet.2004.08.008
M3 - Article
C2 - 15585325
AN - SCOPUS:10044243718
VL - 96
SP - 211
EP - 217
JO - Immunology Letters
JF - Immunology Letters
SN - 0165-2478
IS - 2
ER -