IL-18 enhances the migration ability of murine melanoma cells through the generation of ROI and the MAPK pathway

Min Kyung Jung, Hyun Keun Song, Kyung Eun Kim, Dae Young Hur, Taesung Kim, Saic Bang, Hyunjeong Park, Dae Ho Cho

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Interleukin-18 (IL-18) has multiple effects on various cells that are involved in immune escape of murine melanoma cells and in the inflammatory responses. This study investigated the effect of IL-18 on the ability of murine melanoma cells to migrate by using B16F10 cells and the IL-18 antisense transfectants of B16F10 cells (the B16F10/IL-18 antisense transfectant). The B16F10 cells were more able to migrate than were the B16F10/IL-18 antisense transfectants. An exogenous IL-18 treatment improved the ability of the B16F10/IL-18 antisense transfectant cells to migrate, indicating that IL-18 enhanced the migration ability of melanoma cells. To determine the signaling mechanisms involved in IL-18-enhanced migration, we measured the ROI levels. It was found that the ROI levels were increased by IL-18, and an antioxidant, N-acetyl-l-cystein (NAC), blocked the effect of IL-18 on migration, suggesting the involvement of ROI in the signal transduction of IL-18-enhanced cell migration. IL-18-enhanced cell migration was also reduced by PD98059. In addition, the level of ERK1/2 phosphorylation was markedly increased by treating with exogenous IL-18 at 20 min. These results suggest that IL-18 enhances the ability of melanoma cells to migrate via the generation of ROI and the MAPK pathway.

Original languageEnglish
Pages (from-to)125-130
Number of pages6
JournalImmunology Letters
Volume107
Issue number2
DOIs
Publication statusPublished - 2006 Nov 15

Keywords

  • IL-18
  • MAPK
  • Melanoma
  • Migration
  • ROI

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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