IL-2 production in developing Th1 cells is regulated by heterodimerization of RelA and T-bet and requires T-bet serine residue 508

Sook Hwang Eun, Jeong-Ho Hong, Laurie H. Glimcher

Research output: Contribution to journalArticle

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Abstract

Interleukin (IL)-2 is the predominant cytokine that is produced by naive Th cells in a primary response. It is required for proliferation and differentiation of Th precursor cells into effector cells. Initial high-level IL-2 production is followed by its decline, and the concomitant induction of cytokines that are typical of the differentiated state. Although the factors that are responsible for the early induction of IL-2 are well defined, the mechanisms that are responsible for its down-regulation in later stages of Th development have not been studied as much. Previous work from our laboratory revealed a repressor function for the T-box transcription factor, T-bet, in IL-2 gene transcription. Here, we report that T-bet S508 is required for the optimal repression of IL-2 production in developing Th1 cells. Phosphorylation of T-bet S508 by casein kinase I and glycogen synthase kinase-3 kinases accompanies T-bet's interaction with the RelA nuclear factor-κB transcription factor. Heterodimerization of T-bet and RelA interferes with the binding of RelA to the IL-2 promoter, and hence, transcriptional activation of the IL-2 gene by RelA. JEM

Original languageEnglish
Pages (from-to)1289-1300
Number of pages12
JournalJournal of Experimental Medicine
Volume202
Issue number9
DOIs
Publication statusPublished - 2005 Nov 7
Externally publishedYes

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Th1 Cells
Serine
Interleukin-2
Casein Kinase I
Cytokines
Glycogen Synthase Kinase 3
Transcriptional Activation
Genes
Transcription Factors
Phosphotransferases
Down-Regulation
Phosphorylation

ASJC Scopus subject areas

  • Immunology

Cite this

IL-2 production in developing Th1 cells is regulated by heterodimerization of RelA and T-bet and requires T-bet serine residue 508. / Eun, Sook Hwang; Hong, Jeong-Ho; Glimcher, Laurie H.

In: Journal of Experimental Medicine, Vol. 202, No. 9, 07.11.2005, p. 1289-1300.

Research output: Contribution to journalArticle

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