IL-33 induces Egr-1-dependent TSLP expression via the MAPK pathways in human keratinocytes

Woo In Ryu, Hana Lee, Jin Hee Kim, Hyun Cheol Bae, Hwa Jung Ryu, Sang Wook Son

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disease in which T-helper type 2 (Th2)-type immune responses are dominant. Th2 cytokine, interleukin (IL)-33 and thymic stromal lymphopoietin (TSLP) have been suggested to have an important role in AD. IL-33 is highly expressed in AD, but its role in AD has not yet been fully understood. To further identify the role of IL-33 in AD, we investigated the expression of TSLP induced by IL-33 in keratinocytes. This study revealed that IL-33 induced TSLP expression in human keratinocytes. Early growth response protein 1 (Egr)-1, which is an inflammatory transcriptional factor, is induced by IL-33. IL-33-mediated TSLP induction in keratinocytes was suppressed by treatment with mitogen-activated protein kinase (MAPK) inhibitors or small interfering RNA against Egr-1. Chromatin immunoprecipitation (ChIP) assay indicated the direct involvement of Egr-1 in IL-33-mediated TSLP induction. Taken together, these findings indicate that IL-33 may increase TSLP expression through an Egr-1-dependent mechanism via ERK1/2, JNK and p38 activation in keratinocytes. These data suggest that the IL-33-ERK/JNK/p38/Egr-1/TSLP axis is involved in allergic skin Th2 inflammation, and it may be a novel therapeutic target.

Original languageEnglish
Pages (from-to)857-863
Number of pages7
JournalExperimental Dermatology
Volume24
Issue number11
DOIs
Publication statusPublished - 2015 Nov 1

Fingerprint

Early Growth Response Protein 1
Mitogen-Activated Protein Kinases
Keratinocytes
Atopic Dermatitis
Skin
Interleukin-33
thymic stromal lymphopoietin
Chromatin Immunoprecipitation
Protein Kinase Inhibitors
Skin Diseases
Small Interfering RNA
Chromatin
Assays

Keywords

  • Atopic dermatitis
  • Early growth response protein 1
  • Interleukin-33
  • Mitogen-activated protein kinase
  • Thymic stromal lymphopoietin

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Molecular Biology
  • Dermatology

Cite this

IL-33 induces Egr-1-dependent TSLP expression via the MAPK pathways in human keratinocytes. / Ryu, Woo In; Lee, Hana; Kim, Jin Hee; Bae, Hyun Cheol; Ryu, Hwa Jung; Son, Sang Wook.

In: Experimental Dermatology, Vol. 24, No. 11, 01.11.2015, p. 857-863.

Research output: Contribution to journalArticle

Ryu, Woo In ; Lee, Hana ; Kim, Jin Hee ; Bae, Hyun Cheol ; Ryu, Hwa Jung ; Son, Sang Wook. / IL-33 induces Egr-1-dependent TSLP expression via the MAPK pathways in human keratinocytes. In: Experimental Dermatology. 2015 ; Vol. 24, No. 11. pp. 857-863.
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