Image-guided in situ cancer vaccination with combination of multi-functional nano-adjuvant and an irreversible electroporation technique

Jun Hyeok Han, Yun Young Lee, Ha Eun Shin, Jieun Han, Jeon Min Kang, Chi Pin James Wang, Jung Hoon Park, Se Na Kim, Jong Hwi Yoon, Ho Keun Kwon, Dae Hwan Park, Tae Eun Park, Young Bin Choy, Dong Hyun Kim, Tae Hyung Kim, Junhong Min, Ik Hwan Kim, Chun Gwon Park, Dong Keun Han, Wooram Park

Research output: Contribution to journalArticlepeer-review

Abstract

Cancer immunotherapy is a next-generation treatment strategy; however, its side effects limit its clinical translation. Here, a novel combination of a multi-functional nano-adjuvant (M-NA) prepared with an iron oxide/gold core and a cationic polymer shell via multilayer synthesis with CpG oligodeoxynucleotide (CpG-ODN) electrostatically complexed on its surface, and irreversible electroporation (IRE) technique was developed for effective image-guided in situ cancer vaccination. The M-NA can be retained long-term in the dense tumoral extracellular matrix after intratumoral injection and internalized by antigen-presenting cells (APCs). The IRE can induce immunogenic cell death. Indeed, in a mouse tumor model, the M-NA showed longer tumor retention time than free CpG-ODN. Compared with other treatments, the combined treatment significantly inhibited tumor growth with 100% survival rate for ∼60 days. The therapy induced the activation of cytotoxic lymphocytes and the maturation of APCs in vivo. This treatment could be effective in image-guided local cancer immunotherapy.

Original languageEnglish
Article number121762
JournalBiomaterials
Volume289
DOIs
Publication statusPublished - 2022 Oct

Keywords

  • Cancer immunotherapy
  • Image-guided cancer therapy
  • Irreversible electroporation
  • Multi-functional nano-adjuvant

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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