Imaging of renal and prostate carcinoma with refractive index radiology

Cheol Yong Yoon, Duck Je Sung, Ju-Han Lee, Aeree Kim, Chil Whan Oh, Jung Ho Je, Byung Mook Weon, Seung Kwon Seol, Aram Pyun, Yeukuang Hwu, Giorgio Margaritondo, Kwan Joong Joo, Duck Ki Yoon

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Aim: Having better edge enhancement and penetrating power, refractive index radiology is suitable for the imaging of weakly absorbing objects such as tissue specimens. In this study the potential of refractive index radiology was evaluated for the imaging of renal cell carcinoma (RCC) and prostate cancer (PCA). Methods: Specimens were cut in 3 mm and 4 μm thickness for X-ray radiology and hematoxylin and eosin (HE) staining, respectively. Radiographic images of RCC and PCA were obtained using the synchrotron hard X-rays from the 7B2 beam-line of the Pohang Light Source (PLS). The imaging technique applied was phase-contrast radiology based on the refraction enhancement mechanism. The resulting radiographic images were analyzed in correlation with those of optical microscopy. Results: Using unmonochromatized hard X-rays, it was possible to obtain images with clear edge enhancement and relatively large field of view (6 cm × 6 cm). Even with overlapping signals from thick samples (more than 700-fold thicker than microscopic images), radiographic images clearly showed histological information of organelles in normal kidney such as glomeruli, tubules, and collecting ducts. Histological information of RCC including tumor subtypes and minute changes such as cystic degeneration could be identified without difficulty. The radiographic images of the prostate were comparable with those of low magnification optical microscopy, providing good visualization of normal microstructures such as adenoma, smooth muscle, and normal glands, or differentiation of tiny tumors from surrounding normal tissues. Conclusions: These results suggest the potential of refractive index radiology to provide a new way of imaging biological tissues with low absorption contrast such as RCC and PCA.

Original languageEnglish
Pages (from-to)96-103
Number of pages8
JournalInternational Journal of Urology
Volume14
Issue number2
DOIs
Publication statusPublished - 2007 Feb 1

Fingerprint

Refractometry
Renal Cell Carcinoma
Radiology
Prostate
Carcinoma
Kidney
Prostatic Neoplasms
X-Rays
Microscopy
Synchrotrons
Hematoxylin
Eosine Yellowish-(YS)
Organelles
Adenoma
Smooth Muscle
Neoplasms
Staining and Labeling
Light

Keywords

  • Prostate cancer
  • Refractive index radiology
  • Renal cell carcinoma

ASJC Scopus subject areas

  • Urology

Cite this

Imaging of renal and prostate carcinoma with refractive index radiology. / Yoon, Cheol Yong; Sung, Duck Je; Lee, Ju-Han; Kim, Aeree; Oh, Chil Whan; Je, Jung Ho; Weon, Byung Mook; Seol, Seung Kwon; Pyun, Aram; Hwu, Yeukuang; Margaritondo, Giorgio; Joo, Kwan Joong; Yoon, Duck Ki.

In: International Journal of Urology, Vol. 14, No. 2, 01.02.2007, p. 96-103.

Research output: Contribution to journalArticle

Yoon, CY, Sung, DJ, Lee, J-H, Kim, A, Oh, CW, Je, JH, Weon, BM, Seol, SK, Pyun, A, Hwu, Y, Margaritondo, G, Joo, KJ & Yoon, DK 2007, 'Imaging of renal and prostate carcinoma with refractive index radiology', International Journal of Urology, vol. 14, no. 2, pp. 96-103. https://doi.org/10.1111/j.1442-2042.2007.01614.x
Yoon, Cheol Yong ; Sung, Duck Je ; Lee, Ju-Han ; Kim, Aeree ; Oh, Chil Whan ; Je, Jung Ho ; Weon, Byung Mook ; Seol, Seung Kwon ; Pyun, Aram ; Hwu, Yeukuang ; Margaritondo, Giorgio ; Joo, Kwan Joong ; Yoon, Duck Ki. / Imaging of renal and prostate carcinoma with refractive index radiology. In: International Journal of Urology. 2007 ; Vol. 14, No. 2. pp. 96-103.
@article{86463fdac22b497e9fad776cb9f0c6f5,
title = "Imaging of renal and prostate carcinoma with refractive index radiology",
abstract = "Aim: Having better edge enhancement and penetrating power, refractive index radiology is suitable for the imaging of weakly absorbing objects such as tissue specimens. In this study the potential of refractive index radiology was evaluated for the imaging of renal cell carcinoma (RCC) and prostate cancer (PCA). Methods: Specimens were cut in 3 mm and 4 μm thickness for X-ray radiology and hematoxylin and eosin (HE) staining, respectively. Radiographic images of RCC and PCA were obtained using the synchrotron hard X-rays from the 7B2 beam-line of the Pohang Light Source (PLS). The imaging technique applied was phase-contrast radiology based on the refraction enhancement mechanism. The resulting radiographic images were analyzed in correlation with those of optical microscopy. Results: Using unmonochromatized hard X-rays, it was possible to obtain images with clear edge enhancement and relatively large field of view (6 cm × 6 cm). Even with overlapping signals from thick samples (more than 700-fold thicker than microscopic images), radiographic images clearly showed histological information of organelles in normal kidney such as glomeruli, tubules, and collecting ducts. Histological information of RCC including tumor subtypes and minute changes such as cystic degeneration could be identified without difficulty. The radiographic images of the prostate were comparable with those of low magnification optical microscopy, providing good visualization of normal microstructures such as adenoma, smooth muscle, and normal glands, or differentiation of tiny tumors from surrounding normal tissues. Conclusions: These results suggest the potential of refractive index radiology to provide a new way of imaging biological tissues with low absorption contrast such as RCC and PCA.",
keywords = "Prostate cancer, Refractive index radiology, Renal cell carcinoma",
author = "Yoon, {Cheol Yong} and Sung, {Duck Je} and Ju-Han Lee and Aeree Kim and Oh, {Chil Whan} and Je, {Jung Ho} and Weon, {Byung Mook} and Seol, {Seung Kwon} and Aram Pyun and Yeukuang Hwu and Giorgio Margaritondo and Joo, {Kwan Joong} and Yoon, {Duck Ki}",
year = "2007",
month = "2",
day = "1",
doi = "10.1111/j.1442-2042.2007.01614.x",
language = "English",
volume = "14",
pages = "96--103",
journal = "International Journal of Urology",
issn = "0919-8172",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Imaging of renal and prostate carcinoma with refractive index radiology

AU - Yoon, Cheol Yong

AU - Sung, Duck Je

AU - Lee, Ju-Han

AU - Kim, Aeree

AU - Oh, Chil Whan

AU - Je, Jung Ho

AU - Weon, Byung Mook

AU - Seol, Seung Kwon

AU - Pyun, Aram

AU - Hwu, Yeukuang

AU - Margaritondo, Giorgio

AU - Joo, Kwan Joong

AU - Yoon, Duck Ki

PY - 2007/2/1

Y1 - 2007/2/1

N2 - Aim: Having better edge enhancement and penetrating power, refractive index radiology is suitable for the imaging of weakly absorbing objects such as tissue specimens. In this study the potential of refractive index radiology was evaluated for the imaging of renal cell carcinoma (RCC) and prostate cancer (PCA). Methods: Specimens were cut in 3 mm and 4 μm thickness for X-ray radiology and hematoxylin and eosin (HE) staining, respectively. Radiographic images of RCC and PCA were obtained using the synchrotron hard X-rays from the 7B2 beam-line of the Pohang Light Source (PLS). The imaging technique applied was phase-contrast radiology based on the refraction enhancement mechanism. The resulting radiographic images were analyzed in correlation with those of optical microscopy. Results: Using unmonochromatized hard X-rays, it was possible to obtain images with clear edge enhancement and relatively large field of view (6 cm × 6 cm). Even with overlapping signals from thick samples (more than 700-fold thicker than microscopic images), radiographic images clearly showed histological information of organelles in normal kidney such as glomeruli, tubules, and collecting ducts. Histological information of RCC including tumor subtypes and minute changes such as cystic degeneration could be identified without difficulty. The radiographic images of the prostate were comparable with those of low magnification optical microscopy, providing good visualization of normal microstructures such as adenoma, smooth muscle, and normal glands, or differentiation of tiny tumors from surrounding normal tissues. Conclusions: These results suggest the potential of refractive index radiology to provide a new way of imaging biological tissues with low absorption contrast such as RCC and PCA.

AB - Aim: Having better edge enhancement and penetrating power, refractive index radiology is suitable for the imaging of weakly absorbing objects such as tissue specimens. In this study the potential of refractive index radiology was evaluated for the imaging of renal cell carcinoma (RCC) and prostate cancer (PCA). Methods: Specimens were cut in 3 mm and 4 μm thickness for X-ray radiology and hematoxylin and eosin (HE) staining, respectively. Radiographic images of RCC and PCA were obtained using the synchrotron hard X-rays from the 7B2 beam-line of the Pohang Light Source (PLS). The imaging technique applied was phase-contrast radiology based on the refraction enhancement mechanism. The resulting radiographic images were analyzed in correlation with those of optical microscopy. Results: Using unmonochromatized hard X-rays, it was possible to obtain images with clear edge enhancement and relatively large field of view (6 cm × 6 cm). Even with overlapping signals from thick samples (more than 700-fold thicker than microscopic images), radiographic images clearly showed histological information of organelles in normal kidney such as glomeruli, tubules, and collecting ducts. Histological information of RCC including tumor subtypes and minute changes such as cystic degeneration could be identified without difficulty. The radiographic images of the prostate were comparable with those of low magnification optical microscopy, providing good visualization of normal microstructures such as adenoma, smooth muscle, and normal glands, or differentiation of tiny tumors from surrounding normal tissues. Conclusions: These results suggest the potential of refractive index radiology to provide a new way of imaging biological tissues with low absorption contrast such as RCC and PCA.

KW - Prostate cancer

KW - Refractive index radiology

KW - Renal cell carcinoma

UR - http://www.scopus.com/inward/record.url?scp=33846283407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846283407&partnerID=8YFLogxK

U2 - 10.1111/j.1442-2042.2007.01614.x

DO - 10.1111/j.1442-2042.2007.01614.x

M3 - Article

VL - 14

SP - 96

EP - 103

JO - International Journal of Urology

JF - International Journal of Urology

SN - 0919-8172

IS - 2

ER -