Imiquimod enhances excitability of dorsal root ganglion neurons by inhibiting background (K 2P) and voltage-gated (K v1.1 and K v1.2) potassium channels

Jaekwang Lee, Taekeun Kim, Jinpyo Hong, Junsung Woo, Hyunjung Min, Eunmi Hwang, Sung J. Lee, Changjoon Lee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Imiquimod (IQ) is known as an agonist of Toll-like receptor 7 (TLR7) and is widely used to treat various infectious skin diseases. However, it causes severe itching sensation as its side effect. The precise mechanism of how IQ causes itching sensation is unknown. A recent report suggested a molecular target of IQ as TLR7 expressed in dorsal root ganglion (DRG) neurons. However, we recently proposed a TLR7-independent mechanism, in which the activation of TLR7 is not required for the action of IQ in DRG neurons. To resolve this controversy regarding the involvement of TLR7 and to address the exact molecular identity of itching sensation by IQ, we investigated the possible molecular target of IQ in DRG neurons.Findings: When IQ was applied to DRG neurons, we observed an increase in action potential (AP) duration and membrane resistance both in wild type and TLR7-deficient mice. Based on these results, we tested whether the treatment of IQ has an effect on the activity of K + channels, K v1.1 and K v1.2 (voltage-gated K + channels) and TREK1 and TRAAK (K 2P channels). IQ effectively reduced the currents mediated by both K + channels in a dose-dependent manner, acting as an antagonist at TREK1 and TRAAK and as a partial antagonist at K v1.1 and K v1.2.Conclusions: Our results demonstrate that IQ blocks the voltage-gated K + channels to increase AP duration and K 2P channels to increase membrane resistance, which are critical for the membrane excitability of DRG neurons. Therefore, we propose that IQ enhances the excitability of DRG neurons by blocking multiple potassium channels and causing pruritus.

Original languageEnglish
Article number2
JournalMolecular Pain
Volume8
DOIs
Publication statusPublished - 2012 Jan 11
Externally publishedYes

Fingerprint

imiquimod
Potassium Channels
Spinal Ganglia
Toll-Like Receptor 7
Neurons
Pruritus
Voltage-Gated Potassium Channels
Action Potentials
Membranes
Infectious Skin Diseases

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Molecular Medicine
  • Cellular and Molecular Neuroscience

Cite this

Imiquimod enhances excitability of dorsal root ganglion neurons by inhibiting background (K 2P) and voltage-gated (K v1.1 and K v1.2) potassium channels. / Lee, Jaekwang; Kim, Taekeun; Hong, Jinpyo; Woo, Junsung; Min, Hyunjung; Hwang, Eunmi; Lee, Sung J.; Lee, Changjoon.

In: Molecular Pain, Vol. 8, 2, 11.01.2012.

Research output: Contribution to journalArticle

Lee, Jaekwang ; Kim, Taekeun ; Hong, Jinpyo ; Woo, Junsung ; Min, Hyunjung ; Hwang, Eunmi ; Lee, Sung J. ; Lee, Changjoon. / Imiquimod enhances excitability of dorsal root ganglion neurons by inhibiting background (K 2P) and voltage-gated (K v1.1 and K v1.2) potassium channels. In: Molecular Pain. 2012 ; Vol. 8.
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abstract = "Background: Imiquimod (IQ) is known as an agonist of Toll-like receptor 7 (TLR7) and is widely used to treat various infectious skin diseases. However, it causes severe itching sensation as its side effect. The precise mechanism of how IQ causes itching sensation is unknown. A recent report suggested a molecular target of IQ as TLR7 expressed in dorsal root ganglion (DRG) neurons. However, we recently proposed a TLR7-independent mechanism, in which the activation of TLR7 is not required for the action of IQ in DRG neurons. To resolve this controversy regarding the involvement of TLR7 and to address the exact molecular identity of itching sensation by IQ, we investigated the possible molecular target of IQ in DRG neurons.Findings: When IQ was applied to DRG neurons, we observed an increase in action potential (AP) duration and membrane resistance both in wild type and TLR7-deficient mice. Based on these results, we tested whether the treatment of IQ has an effect on the activity of K + channels, K v1.1 and K v1.2 (voltage-gated K + channels) and TREK1 and TRAAK (K 2P channels). IQ effectively reduced the currents mediated by both K + channels in a dose-dependent manner, acting as an antagonist at TREK1 and TRAAK and as a partial antagonist at K v1.1 and K v1.2.Conclusions: Our results demonstrate that IQ blocks the voltage-gated K + channels to increase AP duration and K 2P channels to increase membrane resistance, which are critical for the membrane excitability of DRG neurons. Therefore, we propose that IQ enhances the excitability of DRG neurons by blocking multiple potassium channels and causing pruritus.",
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AB - Background: Imiquimod (IQ) is known as an agonist of Toll-like receptor 7 (TLR7) and is widely used to treat various infectious skin diseases. However, it causes severe itching sensation as its side effect. The precise mechanism of how IQ causes itching sensation is unknown. A recent report suggested a molecular target of IQ as TLR7 expressed in dorsal root ganglion (DRG) neurons. However, we recently proposed a TLR7-independent mechanism, in which the activation of TLR7 is not required for the action of IQ in DRG neurons. To resolve this controversy regarding the involvement of TLR7 and to address the exact molecular identity of itching sensation by IQ, we investigated the possible molecular target of IQ in DRG neurons.Findings: When IQ was applied to DRG neurons, we observed an increase in action potential (AP) duration and membrane resistance both in wild type and TLR7-deficient mice. Based on these results, we tested whether the treatment of IQ has an effect on the activity of K + channels, K v1.1 and K v1.2 (voltage-gated K + channels) and TREK1 and TRAAK (K 2P channels). IQ effectively reduced the currents mediated by both K + channels in a dose-dependent manner, acting as an antagonist at TREK1 and TRAAK and as a partial antagonist at K v1.1 and K v1.2.Conclusions: Our results demonstrate that IQ blocks the voltage-gated K + channels to increase AP duration and K 2P channels to increase membrane resistance, which are critical for the membrane excitability of DRG neurons. Therefore, we propose that IQ enhances the excitability of DRG neurons by blocking multiple potassium channels and causing pruritus.

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