Immobilization and stabilization of subtilisin Carlsberg in magnetically-separable mesoporous silica for transesterification in an organic solvent

Ee Taek Hwang, Byoungsoo Lee, Meiling Zhang, Seung Hyun Jun, Jongmin Shim, Jinwoo Lee, Jungbae Kim, Man Bock Gu

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Subtilisin Carlsberg (SC) was immobilized and stabilized on magnetically-separable mesoporous silica (Mag-MSU-F) in the form of nanoscale enzyme reactors (NERs) based on the ship-in-a-bottle mechanism. Stabilized NERs of SC (NER-SC) were freeze-dried and successfully used for the transesterification of N-acetyl-l-phenylalanine ethyl ester with n-propanol in isooctane. Magnetic separation of Mag-MSU-F facilitated the repeated usages of stable NER-SC. This is the first demonstration for the use of stable and magnetically-separable NERs in an organic solvent, which has the potential for environmentally-friendly synthesis using enzymes in organic solvents.

Original languageEnglish
Pages (from-to)1884-1887
Number of pages4
JournalGreen Chemistry
Volume14
Issue number7
DOIs
Publication statusPublished - 2012 Jul

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution

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