TY - JOUR
T1 - Immobilization as a technical possibility for long-term storage of bacterial biosensors
AU - Kyeong, Seo Park
AU - Baumstark-Khan, Christa
AU - Rettberg, Petra
AU - Horneck, Gerda
AU - Rabbow, Elke
AU - Man, Bock Gu
N1 - Funding Information:
Acknowledgements This work was performed during a 6-month stay by Mr Kyeong Seo Park at the Radiobiology Department of the DLR-Institute of Aerospace Medicine under the supervision of Dr C. Baumstark-Khan (DLR) and Dr E. Rabbow (RWTH). The work was supported by the German Aerospace Centre (project contract WB9916 given to RWTH) and in part by the National Research Laboratory (2001 NRL) program of the Korean Institute of Science and Technology Evaluation and Planning (project M10104000094_01J000004100). Mr Kyeong Seo Park has been financially supported to visit DLR in Germany by the BK21 program under the Korea Ministry of Education.
PY - 2005/5
Y1 - 2005/5
N2 - For applications in field experiments, the recombinant strain Salmonella typhimurium TA1535 was immobilized to permit its immediate utilization after long storage periods. Salmonella typhimurium TA1535 cells contain the plasmid that has an inducible SOS promoter fused to a promoterless luxCDABFE operon from Photobacterium leiognathi. The induction of bioluminescence occurs in the presence of the DNA-damaging agent mitomycin C which stimulates the bacterial SOS response. Early stationary phase cells were immobilized at a cell concentration of 1010 CFU/ml in microtiter plates and stored up to 6 weeks at 4°C in a sealed container. Even after 4 weeks of storage, the bioluminescence kinetics and yield in response to different concentrations of mitomycin C were not significantly different from those of freshly prepared samples.
AB - For applications in field experiments, the recombinant strain Salmonella typhimurium TA1535 was immobilized to permit its immediate utilization after long storage periods. Salmonella typhimurium TA1535 cells contain the plasmid that has an inducible SOS promoter fused to a promoterless luxCDABFE operon from Photobacterium leiognathi. The induction of bioluminescence occurs in the presence of the DNA-damaging agent mitomycin C which stimulates the bacterial SOS response. Early stationary phase cells were immobilized at a cell concentration of 1010 CFU/ml in microtiter plates and stored up to 6 weeks at 4°C in a sealed container. Even after 4 weeks of storage, the bioluminescence kinetics and yield in response to different concentrations of mitomycin C were not significantly different from those of freshly prepared samples.
UR - http://www.scopus.com/inward/record.url?scp=21244442303&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21244442303&partnerID=8YFLogxK
U2 - 10.1007/s00411-005-0271-1
DO - 10.1007/s00411-005-0271-1
M3 - Article
C2 - 15791471
AN - SCOPUS:21244442303
VL - 44
SP - 69
EP - 71
JO - Radiation and Environmental Biophysics
JF - Radiation and Environmental Biophysics
SN - 0301-634X
IS - 1
ER -