Immobilization as a technical possibility for long-term storage of bacterial biosensors

Seo Park Kyeong, Christa Baumstark-Khan, Petra Rettberg, Gerda Horneck, Elke Rabbow, Man Bock Gu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

For applications in field experiments, the recombinant strain Salmonella typhimurium TA1535 was immobilized to permit its immediate utilization after long storage periods. Salmonella typhimurium TA1535 cells contain the plasmid that has an inducible SOS promoter fused to a promoterless luxCDABFE operon from Photobacterium leiognathi. The induction of bioluminescence occurs in the presence of the DNA-damaging agent mitomycin C which stimulates the bacterial SOS response. Early stationary phase cells were immobilized at a cell concentration of 1010 CFU/ml in microtiter plates and stored up to 6 weeks at 4°C in a sealed container. Even after 4 weeks of storage, the bioluminescence kinetics and yield in response to different concentrations of mitomycin C were not significantly different from those of freshly prepared samples.

Original languageEnglish
Pages (from-to)69-71
Number of pages3
JournalRadiation and Environmental Biophysics
Volume44
Issue number1
DOIs
Publication statusPublished - 2005 May 1
Externally publishedYes

Fingerprint

Bioluminescence
bioluminescence
Salmonella
biosensors
Mitomycin
Biosensing Techniques
Salmonella typhimurium
immobilization
bioinstrumentation
Biosensors
Immobilization
salmonella
mitomycin
Photobacterium
Salmonella Typhimurium
Immobilized Cells
Photobacterium leiognathi
Operon
cells
plasmid

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biophysics
  • Environmental Science(all)
  • Environmental Science (miscellaneous)
  • Environmental Chemistry
  • Radiology Nuclear Medicine and imaging
  • Physics and Astronomy (miscellaneous)
  • Radiation

Cite this

Immobilization as a technical possibility for long-term storage of bacterial biosensors. / Kyeong, Seo Park; Baumstark-Khan, Christa; Rettberg, Petra; Horneck, Gerda; Rabbow, Elke; Gu, Man Bock.

In: Radiation and Environmental Biophysics, Vol. 44, No. 1, 01.05.2005, p. 69-71.

Research output: Contribution to journalArticle

Kyeong, Seo Park ; Baumstark-Khan, Christa ; Rettberg, Petra ; Horneck, Gerda ; Rabbow, Elke ; Gu, Man Bock. / Immobilization as a technical possibility for long-term storage of bacterial biosensors. In: Radiation and Environmental Biophysics. 2005 ; Vol. 44, No. 1. pp. 69-71.
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