Abstract
For applications in field experiments, the recombinant strain Salmonella typhimurium TA1535 was immobilized to permit its immediate utilization after long storage periods. Salmonella typhimurium TA1535 cells contain the plasmid that has an inducible SOS promoter fused to a promoterless luxCDABFE operon from Photobacterium leiognathi. The induction of bioluminescence occurs in the presence of the DNA-damaging agent mitomycin C which stimulates the bacterial SOS response. Early stationary phase cells were immobilized at a cell concentration of 1010 CFU/ml in microtiter plates and stored up to 6 weeks at 4°C in a sealed container. Even after 4 weeks of storage, the bioluminescence kinetics and yield in response to different concentrations of mitomycin C were not significantly different from those of freshly prepared samples.
| Original language | English |
|---|---|
| Pages (from-to) | 69-71 |
| Number of pages | 3 |
| Journal | Radiation and Environmental Biophysics |
| Volume | 44 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2005 May 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Agricultural and Biological Sciences (miscellaneous)
- Biophysics
- Environmental Science(all)
- Environmental Science (miscellaneous)
- Environmental Chemistry
- Radiology Nuclear Medicine and imaging
- Physics and Astronomy (miscellaneous)
- Radiation
Cite this
Immobilization as a technical possibility for long-term storage of bacterial biosensors. / Kyeong, Seo Park; Baumstark-Khan, Christa; Rettberg, Petra; Horneck, Gerda; Rabbow, Elke; Gu, Man Bock.
In: Radiation and Environmental Biophysics, Vol. 44, No. 1, 01.05.2005, p. 69-71.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immobilization as a technical possibility for long-term storage of bacterial biosensors
AU - Kyeong, Seo Park
AU - Baumstark-Khan, Christa
AU - Rettberg, Petra
AU - Horneck, Gerda
AU - Rabbow, Elke
AU - Gu, Man Bock
PY - 2005/5/1
Y1 - 2005/5/1
N2 - For applications in field experiments, the recombinant strain Salmonella typhimurium TA1535 was immobilized to permit its immediate utilization after long storage periods. Salmonella typhimurium TA1535 cells contain the plasmid that has an inducible SOS promoter fused to a promoterless luxCDABFE operon from Photobacterium leiognathi. The induction of bioluminescence occurs in the presence of the DNA-damaging agent mitomycin C which stimulates the bacterial SOS response. Early stationary phase cells were immobilized at a cell concentration of 1010 CFU/ml in microtiter plates and stored up to 6 weeks at 4°C in a sealed container. Even after 4 weeks of storage, the bioluminescence kinetics and yield in response to different concentrations of mitomycin C were not significantly different from those of freshly prepared samples.
AB - For applications in field experiments, the recombinant strain Salmonella typhimurium TA1535 was immobilized to permit its immediate utilization after long storage periods. Salmonella typhimurium TA1535 cells contain the plasmid that has an inducible SOS promoter fused to a promoterless luxCDABFE operon from Photobacterium leiognathi. The induction of bioluminescence occurs in the presence of the DNA-damaging agent mitomycin C which stimulates the bacterial SOS response. Early stationary phase cells were immobilized at a cell concentration of 1010 CFU/ml in microtiter plates and stored up to 6 weeks at 4°C in a sealed container. Even after 4 weeks of storage, the bioluminescence kinetics and yield in response to different concentrations of mitomycin C were not significantly different from those of freshly prepared samples.
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UR - http://www.scopus.com/inward/citedby.url?scp=21244442303&partnerID=8YFLogxK
U2 - 10.1007/s00411-005-0271-1
DO - 10.1007/s00411-005-0271-1
M3 - Article
VL - 44
SP - 69
EP - 71
JO - Radiation and Environmental Biophysics
JF - Radiation and Environmental Biophysics
SN - 0301-634X
IS - 1
ER -