Immobilization of acetyl xylan esterase on modified graphite oxide and utilization to peracetic acid production

Hah Young Yoo, Ja Hyun Lee, Young Joon Suh, Sung Bong Kim, Seung Moon Park, Seung Wook Kim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In the previous work, acetyl xylan esterase (AXE) of Aspergillus ficcum was successfully produced in Pichia pastoris as host. In this study, the recombinant AXE was immobilized on graphite oxide and used for the production of peracetic acid. Immobilization efficiency was enhanced by modifying graphite oxide via surface functionalization. The conditions for enzyme immobilization were also investigated and the optimal conditions were determined as 4℃ of temperature, 24 h of reaction time and pH 7. The activity of immobilized AXE was found to be 62.53 U/g-support. With the immobilized AXE, about 134 mM of peracetic acid was produced under 37℃ of temperature and 30 min of reaction time. Enzyme activity remained at > 50% of the original after 10 production cycles.

Original languageEnglish
Pages (from-to)1042-1047
Number of pages6
JournalBiotechnology and Bioprocess Engineering
Volume19
Issue number6
DOIs
Publication statusPublished - 2015 Jan 1

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Keywords

  • acetyl xylan esterase
  • enzyme immobilization
  • graphite oxide
  • peracetic acid

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Biomedical Engineering
  • Bioengineering

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