Immobilization of GL-7-ACA Acylase for the Production of 7-ACA

Seung Kwon Lee, Seung Won Park, Yong In Kim, Koo Hun Chung, Suk In Hong, Seung Wook Kim

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Glutaryl-7-aminocephalosporanic acid(GL-7-ACA) acylase is an important enzyme for the production of 7-ACA (7-aminocephalosporanic acid). For an efficient immobilization of GL-7-ACA acylase, various carriers were tested. A high-porous hydrophilic carrier (FPHA) among various carriers tested was found to be the best for the im-mobilization of GL-7-ACA acylase. In order to develop an effective immobilization method of GL-7-ACA acylase, the parameters that affect the immobilization of GL-7-ACA acylase were also investigated under different conditions of buffer solution and different concentrations of glutaraldehyde. The highest value of GL-7-ACA acylase activity (70 Unit/g-matrix) was obtained when immobilized with 1% glutaraldehyde in a 0.1 M Tris buffer (pH 8.0). Also, in order to enhance the activity of the immobilized GL-7-ACA acylase, unreacted aldehyde groups were quenched by reaction with a low molecular weight agent such as L-lysine after immobilization. The highest activity of immobilized GL7-ACA acylase was obtained at 0.1% of L-lysine. The immobilized GL-7-ACA acylase was tested for long-term stability and it was found that the activity was retained at about 62% of the initial value after 72 times of reuse at 25 °C.

Original languageEnglish
Pages (from-to)261-266
Number of pages6
JournalKorean Journal of Chemical Engineering
Volume19
Issue number2
Publication statusPublished - 2002 Mar 1

Fingerprint

amidase
Acids
Glutaral
Lysine
7-aminocephalosporanic acid
glutaryl-7-aminocephalosporanic acid
Tromethamine
Aldehydes

Keywords

  • 7-ACA
  • FPHA
  • GL-7-ACA Acylase
  • Immobilization

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Lee, S. K., Park, S. W., Kim, Y. I., Chung, K. H., Hong, S. I., & Kim, S. W. (2002). Immobilization of GL-7-ACA Acylase for the Production of 7-ACA. Korean Journal of Chemical Engineering, 19(2), 261-266.

Immobilization of GL-7-ACA Acylase for the Production of 7-ACA. / Lee, Seung Kwon; Park, Seung Won; Kim, Yong In; Chung, Koo Hun; Hong, Suk In; Kim, Seung Wook.

In: Korean Journal of Chemical Engineering, Vol. 19, No. 2, 01.03.2002, p. 261-266.

Research output: Contribution to journalArticle

Lee, SK, Park, SW, Kim, YI, Chung, KH, Hong, SI & Kim, SW 2002, 'Immobilization of GL-7-ACA Acylase for the Production of 7-ACA', Korean Journal of Chemical Engineering, vol. 19, no. 2, pp. 261-266.
Lee SK, Park SW, Kim YI, Chung KH, Hong SI, Kim SW. Immobilization of GL-7-ACA Acylase for the Production of 7-ACA. Korean Journal of Chemical Engineering. 2002 Mar 1;19(2):261-266.
Lee, Seung Kwon ; Park, Seung Won ; Kim, Yong In ; Chung, Koo Hun ; Hong, Suk In ; Kim, Seung Wook. / Immobilization of GL-7-ACA Acylase for the Production of 7-ACA. In: Korean Journal of Chemical Engineering. 2002 ; Vol. 19, No. 2. pp. 261-266.
@article{dbee74b4d3cb4e268aa3958e4f875832,
title = "Immobilization of GL-7-ACA Acylase for the Production of 7-ACA",
abstract = "Glutaryl-7-aminocephalosporanic acid(GL-7-ACA) acylase is an important enzyme for the production of 7-ACA (7-aminocephalosporanic acid). For an efficient immobilization of GL-7-ACA acylase, various carriers were tested. A high-porous hydrophilic carrier (FPHA) among various carriers tested was found to be the best for the im-mobilization of GL-7-ACA acylase. In order to develop an effective immobilization method of GL-7-ACA acylase, the parameters that affect the immobilization of GL-7-ACA acylase were also investigated under different conditions of buffer solution and different concentrations of glutaraldehyde. The highest value of GL-7-ACA acylase activity (70 Unit/g-matrix) was obtained when immobilized with 1{\%} glutaraldehyde in a 0.1 M Tris buffer (pH 8.0). Also, in order to enhance the activity of the immobilized GL-7-ACA acylase, unreacted aldehyde groups were quenched by reaction with a low molecular weight agent such as L-lysine after immobilization. The highest activity of immobilized GL7-ACA acylase was obtained at 0.1{\%} of L-lysine. The immobilized GL-7-ACA acylase was tested for long-term stability and it was found that the activity was retained at about 62{\%} of the initial value after 72 times of reuse at 25 °C.",
keywords = "7-ACA, FPHA, GL-7-ACA Acylase, Immobilization",
author = "Lee, {Seung Kwon} and Park, {Seung Won} and Kim, {Yong In} and Chung, {Koo Hun} and Hong, {Suk In} and Kim, {Seung Wook}",
year = "2002",
month = "3",
day = "1",
language = "English",
volume = "19",
pages = "261--266",
journal = "Korean Journal of Chemical Engineering",
issn = "0256-1115",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - Immobilization of GL-7-ACA Acylase for the Production of 7-ACA

AU - Lee, Seung Kwon

AU - Park, Seung Won

AU - Kim, Yong In

AU - Chung, Koo Hun

AU - Hong, Suk In

AU - Kim, Seung Wook

PY - 2002/3/1

Y1 - 2002/3/1

N2 - Glutaryl-7-aminocephalosporanic acid(GL-7-ACA) acylase is an important enzyme for the production of 7-ACA (7-aminocephalosporanic acid). For an efficient immobilization of GL-7-ACA acylase, various carriers were tested. A high-porous hydrophilic carrier (FPHA) among various carriers tested was found to be the best for the im-mobilization of GL-7-ACA acylase. In order to develop an effective immobilization method of GL-7-ACA acylase, the parameters that affect the immobilization of GL-7-ACA acylase were also investigated under different conditions of buffer solution and different concentrations of glutaraldehyde. The highest value of GL-7-ACA acylase activity (70 Unit/g-matrix) was obtained when immobilized with 1% glutaraldehyde in a 0.1 M Tris buffer (pH 8.0). Also, in order to enhance the activity of the immobilized GL-7-ACA acylase, unreacted aldehyde groups were quenched by reaction with a low molecular weight agent such as L-lysine after immobilization. The highest activity of immobilized GL7-ACA acylase was obtained at 0.1% of L-lysine. The immobilized GL-7-ACA acylase was tested for long-term stability and it was found that the activity was retained at about 62% of the initial value after 72 times of reuse at 25 °C.

AB - Glutaryl-7-aminocephalosporanic acid(GL-7-ACA) acylase is an important enzyme for the production of 7-ACA (7-aminocephalosporanic acid). For an efficient immobilization of GL-7-ACA acylase, various carriers were tested. A high-porous hydrophilic carrier (FPHA) among various carriers tested was found to be the best for the im-mobilization of GL-7-ACA acylase. In order to develop an effective immobilization method of GL-7-ACA acylase, the parameters that affect the immobilization of GL-7-ACA acylase were also investigated under different conditions of buffer solution and different concentrations of glutaraldehyde. The highest value of GL-7-ACA acylase activity (70 Unit/g-matrix) was obtained when immobilized with 1% glutaraldehyde in a 0.1 M Tris buffer (pH 8.0). Also, in order to enhance the activity of the immobilized GL-7-ACA acylase, unreacted aldehyde groups were quenched by reaction with a low molecular weight agent such as L-lysine after immobilization. The highest activity of immobilized GL7-ACA acylase was obtained at 0.1% of L-lysine. The immobilized GL-7-ACA acylase was tested for long-term stability and it was found that the activity was retained at about 62% of the initial value after 72 times of reuse at 25 °C.

KW - 7-ACA

KW - FPHA

KW - GL-7-ACA Acylase

KW - Immobilization

UR - http://www.scopus.com/inward/record.url?scp=0036267851&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036267851&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0036267851

VL - 19

SP - 261

EP - 266

JO - Korean Journal of Chemical Engineering

JF - Korean Journal of Chemical Engineering

SN - 0256-1115

IS - 2

ER -