Immobilization of glucose oxidase onto cobalt based on silica core/shell nanoparticles as carrier

Hee Uk Lee, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Co-B/SiO 2/NH 2 magnetic nanoparticles (NPs) were prepared from a silica shell-coated Co-B core using the Stöber method and amine-modification on the surface. Glucose oxidase (GOD) was covalently immobilized on the surface of Co-B/SiO 2/NH 2 NPs using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) as an activating agent. The magnetic NPs characteristics, such as the synthesis of Co-B/SiO 2/NH 2 NPs, effect of pH, temperature, and concentration of buffer for enzyme immobilization, were investigated. The optimal reaction conditions for immobilization were determined to be 0.1 M of phosphate buffer solution, pH 7.0, and 5°C. In the case of immobilized GOD without d-glucose and with 0.1 M of d-glucose for blocking, 22.98 U/g and 24.83 U/g of their original activity were retained after 7 reuses, respectively.

Original languageEnglish
Pages (from-to)1282-1286
Number of pages5
JournalProcess Biochemistry
Volume47
Issue number8
DOIs
Publication statusPublished - 2012 Aug 1

Fingerprint

Glucose Oxidase
Glucose oxidase
Cobalt
Silicon Dioxide
Immobilization
Nanoparticles
Silica
Glucose
Buffers
Enzyme immobilization
Amines
Phosphates
Temperature
Enzymes

Keywords

  • Co-B/SiO
  • Core/shell
  • Glucose oxidase
  • Immobilization
  • Nanoparticles

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Immobilization of glucose oxidase onto cobalt based on silica core/shell nanoparticles as carrier. / Lee, Hee Uk; Park, Chulhwan; Kim, Seung Wook.

In: Process Biochemistry, Vol. 47, No. 8, 01.08.2012, p. 1282-1286.

Research output: Contribution to journalArticle

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N2 - Co-B/SiO 2/NH 2 magnetic nanoparticles (NPs) were prepared from a silica shell-coated Co-B core using the Stöber method and amine-modification on the surface. Glucose oxidase (GOD) was covalently immobilized on the surface of Co-B/SiO 2/NH 2 NPs using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) as an activating agent. The magnetic NPs characteristics, such as the synthesis of Co-B/SiO 2/NH 2 NPs, effect of pH, temperature, and concentration of buffer for enzyme immobilization, were investigated. The optimal reaction conditions for immobilization were determined to be 0.1 M of phosphate buffer solution, pH 7.0, and 5°C. In the case of immobilized GOD without d-glucose and with 0.1 M of d-glucose for blocking, 22.98 U/g and 24.83 U/g of their original activity were retained after 7 reuses, respectively.

AB - Co-B/SiO 2/NH 2 magnetic nanoparticles (NPs) were prepared from a silica shell-coated Co-B core using the Stöber method and amine-modification on the surface. Glucose oxidase (GOD) was covalently immobilized on the surface of Co-B/SiO 2/NH 2 NPs using N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) as an activating agent. The magnetic NPs characteristics, such as the synthesis of Co-B/SiO 2/NH 2 NPs, effect of pH, temperature, and concentration of buffer for enzyme immobilization, were investigated. The optimal reaction conditions for immobilization were determined to be 0.1 M of phosphate buffer solution, pH 7.0, and 5°C. In the case of immobilized GOD without d-glucose and with 0.1 M of d-glucose for blocking, 22.98 U/g and 24.83 U/g of their original activity were retained after 7 reuses, respectively.

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